2-ethylhexyl 10-ethyl-4-[[2-[(2-ethylhexyl)oxy]-2-oxoethyl]thio]-7-oxo-8-oxa-3,5-dithia-4-phosphatetradecanoate 4-oxide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Qualifier:

according to guideline

Guideline:

other: EDI-Richtlinien für die Untersuchung von Abwasser, 1974

Deviations:

yes

Remarks:

Carried out on the computerized BAROMAT apparatus. Test substance was not water soluble; stable suspensions were prepared.

GLP compliance:

not specified

Specific details on test material used for the study:

TEST MATERIAL (as stated in study report): TK 12 184; Trade name D 16 - 51

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. Mixt. 4/5/6

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room-temperature (20 - 23 deg C)

Oxygen conditions:

aerobic

Inoculum or test system:

other: waste water

Details on inoculum:

Polyvalent waste-water bacteria collected on June 18, 1981, in the aeration unit of the sewage treatment plant of Rheinfelden, Switzerland.

Duration of test (contact time):

5 d

Initial conc.:

5 mg/L

Based on:

test mat.

Initial conc.:

10 mg/L

Based on:

test mat.

Initial conc.:

20 mg/L

Based on:

test mat.

Initial conc.:

40 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Temperature: 20 +/- 1 deg C
Lighting: darkness (water-bath)

Reference substance:

benzoic acid, sodium salt

Parameter:

other: BOD5

Remarks on result:

other: see results below

Parameter:

BOD5

Value:

7.6 other: mg O2/L

Remarks on result:

other: 5 mg/L TK 12 184 consumed 7.6 mg oxygen/L = 88.4 % of the Theoretical Oxygen Demand (BODT).

Remarks:

At concentrations of 10, 20 and 40 mg/L TK 12 194, the oxygen demand was below the range of an accurate measurement.

Parameter:

ThOD

Value:

8.6 other: 8.6 mg O2/L

Remarks on result:

other: calculated

Remarks:

according to OECD Method No. 301D, 1980

Results with reference substance:

20 mg/L Sodium benzoate used as a reference substance in this test consumed 24.4 mg O2/L equal to 76.3 % of the
theoretical oxygen demand.

Conclusions:

Under these test conditions, 5 mg/L TK 12 184 consumed 7.6 mg oxygen/L = 88.4 % of the Theoretical Oxygen Demand (BODT). At concentrations of 10, 20 and 40 mg/L TK 12 184, the oxygen demand was below the range of an accurate measurement.

Reference 2

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung, Verlag Chemie Weinheim (1979). Method H 41 - 1, pages 1-6, DIN 38 409, 9. Lieferung, Ausgabe 1981

Deviations:

not specified

GLP compliance:

not specified

Specific details on test material used for the study:

TEST MATERIAL (as stated in study report): TK 12 184; trade name D 16 - 51

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. Mixt. 4/5/6

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 20 - 24 deg C

Inoculum or test system:

other: Potassiumbichromate as oxidizing agent

Duration of test (contact time):

110 min

Initial conc.:

170 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

other: COD

Details on study design:

Vessels: 250 ml COD - flasks with reflux condenser
Oxidizing agent: 10 ml K2Cr207
pH: <1
Tempertaure 148 + 30 deg C / Reflux

Parameter:

other: COD

Remarks on result:

other: see results below

Parameter:

COD

Value:

1 559.4 mg O2/g test mat.

Remarks on result:

other: S.D. = 0.15%

Conclusions:

Under these standard test conditions, the Chemical Oxygen Demand (COD) for TK 12 184 was 1559.40 mg Oxygen / g TK 12 184 (Standard Deviation 0.15 %)

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1985

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

1981

Deviations:

yes

Remarks:

Volume of test solution was 1.5 liters instead of 3.0 liters.

GLP compliance:

yes

Specific details on test material used for the study:

TEST MATERIAL (as stated in study report): TK 12184; trade name D 16-051

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. 312-187
- Expiration date of the lot/batch: 12/1985
- Purity: commercial grade

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Oxygen conditions:

aerobic

Inoculum or test system:

other: Bacteria collected fron activated sludge of the sewage treatment plant of CH-4153 Reinach on O2/01/85

Details on inoculum:

Preparation was carried out according to the method described in the guideline.

Duration of test (contact time):

28 d

Initial conc.:

10 mg/L

Based on:

test mat.

Initial conc.:

20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Vessels: 2 liters flasks equipped with gas inlet and magnetic stirrer.
Water: The test medium was prepared according to the method described in the guideline.
Temperature: 22 + or - 2 degrees
Air: Approx. 50 mL/min free of carbon dioxide.

Reference substance:

aniline

Parameter:

% degradation (CO2 evolution)

Value:

11

Sampling time:

28 d

Remarks on result:

other: at 10 mg/L concentration

Parameter:

% degradation (CO2 evolution)

Value:

12

Sampling time:

28 d

Remarks on result:

other: at 20 mg/L concentration

The biodegradation of the reference substance aniline (20 mg/L) was calculated as 100 % in 28 days

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Under these guideline test condition (OECD 301B), TK 12 184 was not readily biodegradable. At concentrations of 10 or 20 mg/L, 11 and 12 percent degradation, respectively, was achieved within 28 days.

Reference 4

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

(Q)SAR

Adequacy of study:

supporting study

Study period:

2018

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Principles of method if other than guideline:

Predicted by US EPA model BIOWIN v4.10

GLP compliance:

no

Details on results:

Biowin1 (Linear Model Prediction) : Biodegrades Fast
Biowin2 (Non-Linear Model Prediction): Biodegrades Fast
Biowin3 (Ultimate Biodegradation Timeframe): Weeks
Biowin4 (Primary Biodegradation Timeframe): Hours
Biowin5 (MITI Linear Model Prediction) : Readily Degradable
Biowin6 (MITI Non-Linear Model Prediction): Not Readily Degradable
Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast
Ready Biodegradability Prediction: YES

Validity criteria fulfilled:

not applicable

Interpretation of results:

readily biodegradable

Conclusions:

The US EPA BIOWIN v4.10 model predicts TGEPO (CAS No. 83547-95-9) to be readily biodegradable.

Reference 5

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): domestic wastewater treatment plant (sewage works, Darmstadt, Germany).
- Preparation of inoculum for exposure: centrifugation, resuspension of solid mterial in tap water (repeat three (3) time).
- Pretreatment: Aliqout of wet sludge material was weighed, dried, and the ratio of wet sludge material to dry weight was determined.
- Concentration of sludge: Aliqouts corresponding to 1.5 g dry sludge material were mixed with one (1) liter of test water (equals 1.5 g/L).

Duration of test (contact time):

28 d

Initial conc.:

102 g/L

Based on:

ThOD

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

Calculated from the decrease of pressure in the reaction vessel.

Details on study design:

Principle: The test flasks prepared according paragraph 6.6 were incubated at 22 ± 2 °C. The pressure decrease in the reaction vessels was measured over complete experimental phase of 28 days using the BSB Sensomat system, Aqualytic Dortmund. The test flasks were closed gastight by a measuring head. Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel.

Identification: Each test unit was uniquely identified with the study number, treatment and replicate number.

Reference substance:

benzoic acid, sodium salt

Test performance:

Inoculum Control: The oxygen demand of the inoculum control (medium and inoculum) was 20 mg O2/L and thus not greater than 60 mg O2/L within 28 days as required by the test guideline.
pH-Value:The pH-value of the test item flasks at the end of the test was within the range of pH 6.0 to 8.5 as required by the test guideline.
Reference Item: The percentage degradation of the reference item should reach the level for ready biodegradability (>60%) within 14 days as required by the test guideline.
The reference item sodium benzoate was degraded to more than 60% after 4 days of incubation.
Test Item: The difference of duplicate values for the degradation of the test item at the plateau, at the end of the test and at the end of the 10-day window should be less than 20%. The validity criterion was fulfilled.

Parameter:

% degradation (O2 consumption)

Value:

22

Sampling time:

3 d

Parameter:

% degradation (O2 consumption)

Value:

66

Sampling time:

10 d

Parameter:

% degradation (O2 consumption)

Value:

82

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The degradation rate of 2-Ethylhexyl Thioglycolate reached 60% within the 10-day window and after 28 days of incubation. According to the test guideline, 2-Ethylhexyl Thioglycolate is considered to be readily biodegradable.

Description of key information

Under guideline test condition (OECD 301B), TK 12 184 was not readily biodegradable at concentrations of 10 or 20 mg/L, achieving 11 and 12 percent degradation, respectively, within 28 days. In a BOD5 study, 5 mg/L TK 12 184 consumed 7.6 mg oxygen/L = 88.4 % of the Theoretical Oxygen Demand (BODT).  At concentrations of 10, 20 and 40 mg/L TK 12 184, the oxygen demand was below the range of an accurate measurement. These results indicate that TK 12 184 is readily biodegradable at concentrations below those employed in the OECD 301B test.

Under standard test conditions, the Chemical Oxygen Demand (COD) for TK 12 184 was determined to be 1559.40 mg Oxygen / g TK 12 184 (Standard Deviation 0.15 %).

The US EPA BIOWIN v4.10 model predicts this substance to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater

Additional information

TGEPO was not shown to be readily biodegradable at concentrations of 10 or 20 mg/L. BOD5 results demonstrate ready biodegradability at a concentration of 5 mg/L, and indicate possible inhibition of the inoculum at higher test concentrations. Under standard test conditions, the Chemical Oxygen Demand (COD) for TK 12 184 was determined to be 1559.40 mg Oxygen / g TK 12 184 (Standard Deviation 0.15 %).  While these results do not fully meet the ready biodegradability criteria, the weight of available evidence indicates that TK 12 184 is at least inherently biodegradable and not persistent. This is further supported by the US EPA BIOWIN v4.10 model, which predicts this substance to be readily biodegradable.