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EC number: 226-109-5 | CAS number: 5281-04-9
The subjects were given five 48 hour covered patch tests with the TS,(each separated by a 24 hour period with aqueous sodium lauryl sulphate).
A patch test was conducted on 25 healthy subjects to assess the skin sensitization potential of test chemicalD&C Red 7/ Lithol rubine BK (CAS No:5281-04-9).
In induction phase, eachsubjects were given five 48 hour covered patch test of 50% lithol rubine BK mixed with talc (each separated by a 24 hour period with aqueous sodium lauryl sulphate).
After a rest period of 10-14 days, the subjects were challenged for 48 hours using the same concentration and later observed for allergic reactions.
Since none of the treated volunteer had developed skin sensitization, the test chemicalD&C Red 7/ Lithol rubine BK (CAS No:5281-04-9) was considered to be not sensitizing to the skin of treated volunteers.
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