Trimanganese bis(orthophosphate)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 140 mg/L
- Sample storage conditions before analysis: Solutions were acidified with HNO3 for stabilisation, samples were analysed immediately after sampling or (if immeidate anaysis not possible) stored at 4°C.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a WAF according to OECD guidance No. 23 was prepared by adding the required amount of test substance to the test medium. The aqueous phase was separated by filtering through a 0.2 µm bottle top filter.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: Test facility bred. Origin: West Aquarium GmbH, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 2.0 ± 1.0 cm
- Weight at study initiation (mean and range, SD): 0.068 - 0.097 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: ad libitum, brine shrimp (Artemia salina) nauplii, and ground flake food TetraMin® (Tetra Werke, Melle, Germany)
- Feeding frequency: once daily
- Health during acclimation (any mortality observed): mortality < 5%

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

1.2 mmol/L

Test temperature:

23 °C

pH:

8.14 - 8.51

Dissolved oxygen:

91 - 109%

Nominal and measured concentrations:

Nominal test substance concentration: 140 mg test substance /L (corresponding to 53.3 mg Mn/L)
Analytically measured substance concentration: 2.0 mg Mn/L (corresponding to 5.3 mg test substance/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (12L)
- Fill volume: 10 L
- Aeration: slight aeration via glass capillaries
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.08 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified tap water
- Total organic carbon: 1.3560 mg/L (NPOC)
- Chlorine: < 0.02 mg/L
- Alkalinity: 1.8 mmol/L
- Conductivity: 222 [µS/cm]


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 3, 24, 48 72 and 96 h

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 60, 120 and 240 mg test item/L
- Results used to determine the conditions for the definitive study: no mortality observed

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 5.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Remarks:

recaluclated from measured Mn concentration

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no

Reported statistics and error estimates:

No statistical analysis was performed as no mortality was detected throughout the study.

Description of key information

Not acutely toxic to fish.

Additional information

The acute toxicity of trimanganese bis(orthophosphate) to fish was investigated in a limit test following OECD guideline 203. Ten fish of the species Danio rerio were exposed to a nominal test substance concentration of 140 mg/L. The test solution was prepared by applying the Water Accommodated Fraction (WAF) approach according to the OECD guidance No. 23 and 29. The concentrations of trimanganese bis(orthophosphate) in the test media were determined by chemical analyses of manganese in the aqueous phase at the start and at the end of the test using ICP-OES (LOQ 9.78 μg Mn/L). The test substance concentrations were recalculated from the measured manganese concentrations. The test was terminated after an exposure period of 96 hours. Mortality or symptoms of intoxication were not observed. Thus a LC50(96 h) value > 5.3 mg/l (mean measured) was reported (LC50(96h)>140 mg/L (nominal)).Based on the experimental results trimanganese bis(orthophosphate) is not acutely toxic to fish.