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EC number: 280-676-3 | CAS number: 83733-23-7
In a stepwise procedure, increasing volumes of demineralized water were added at room temperature to a defined amount of the test item. After each addition of an amount of water the mixture was treated with ultrasound for 15 minutes and visually checked for any undissolved particles.
Table4: Results of the preliminary test
Amount of test item
Added volume of demineralized water / mL
Total volume of demineralized water / mL
Appearance of mixture
The preliminary test showed that the water solubility of the test item is > 10 mg/L. According to the preliminary test the water solubility was determined by the flask method in the main test.
The flask method was performed as described above. The data for the evaluation are given in Table5. After the equilibration time precipitate was observed in the flasks of the experiments excluding in the blank experiment.Asthe resulting solutions of the experiments were too concentrated for pipetting aliquots were weighted in graduated flasks and filled up to 10 mL with demin. water. Therefore the measured density of 1.14 was used to calculate the volume of the aliquots of the flasks. The water solubility was calculated from the measured and unrounded concentration values and not from the rounded values given in Table5.
Table5: Water solubility experiments of the test item
24 h experiment
48 h experiment
72 h experiment
168 h experiment
experiment (72 h)
Amount of test item / g
Amount of demineralized water / mL
Stirring time at 30 °C / h
pH at 24 °C
Aliquots of the flasks / mg
Aliquots of the flasks / mL
1st Dilution / mL
Aliquot of the flasks / mL
2nd Dilution / mL
Measured concentration / mg/L
137.48 / 136.99
162.36 / 162.24
96.69 / 96.58
73.30 / 72.94
-- / --
Mean concentration / mg/L
Water solubility / g/L
The water solubilities showed a decreasing tendency. As a conservative approach the highest value was stated. The water solubility of the test item at 20 °C was determined to be 507 g/L.
The water solubility cS of the test item Acid Orange 166 at a temperature of 20 °C was determined according to the flask method. It was found to be without correction of the purity:
cS = 507 g/L
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