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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Remarks:
intraperitoneal
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1974/75
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report date:
1976

Materials and methods

Principles of method if other than guideline:
BASF-Test
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, bromo derivatives
EC Number:
215-516-3
EC Name:
Anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, bromo derivatives
Cas Number:
1328-18-3
Molecular formula:
C34H14Br2O2
IUPAC Name:
anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, bromo derivatives
Test material form:
solid
Details on test material:
Vat Blue 19

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
CMC (carboxymethyl cellulose)
Remarks:
0.5% aqueous solution
Details on exposure:
Concentration in vehicle: 1.5 to 35%
Doses:
no data
No. of animals per sex per dose:
no data
Control animals:
no
Details on study design:
no data
Statistics:
NA

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
250 mg/kg bw
Based on:
test mat.
Remarks on result:
other: intraperitoneal in mouse
Mortality:
No data
Clinical signs:
stagger, spastic movements, dyspnea, apathy, paresis of the rear extremities
Body weight:
A body weight loss at the beginning of experiment was reported.
Gross pathology:
intradominal fluids

Applicant's summary and conclusion

Conclusions:
In an acute intraperitoneal toxicity study, the LD50 of the test item was determined to be approximately 250 mg/kg bw in mice.
Executive summary:

In an acute intraperitoneal toxicity study according to BASF method, mice were given a single oral dose of 1.5 - 35 % suspension of the test item diluted in 0.5% aqueous carboxymethylcellulose and were observed for 7 days.

All animals survived until the end of the study. A body weight loss at the beginning of experiment was reported. Adverse clinical signs of toxicity were observed, such as stagger, spastic movements, dyspnea, apathy, paresis of the rear extremities. Moreover, gross pathological findings were intradominal fluids. Based on the results from this study, the LD50 of the test item was determined to be approximately 250 mg/kg bw in mice.