Registration Dossier
Registration Dossier
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EC number: 210-291-8 | CAS number: 612-12-4
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
- Principles of method if other than guideline:
- Ames-Test
- GLP compliance:
- no
Test material
Method
- Target gene:
- In the Salmonella typhimurium strains (TA 1535, TA 100, TA 1537, TA 98) the amino acid histidine locus is the target gene.
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S-9 mix
- Test concentrations with justification for top dose:
- 10, 50, 100 and 500 µg/plate for TA 1535, TA 1537 and TA 98
10, 50, 10, 200 and 300 µg/plate for TA 100 because 500 µg/plate proved already to be toxic to this strain.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Additional information on results:
- TA 100 responded with a concentration-dependent increase in revertants with and without metabolic activation. With metabolic activation the effect on TA 100 was significantly enhanced.
Applicant's summary and conclusion
- Conclusions:
- It can be stated that 1,2-Bis(chloromethyl)benzene is mutagenic under the conditions of the applied bacterial test system.
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