(2-hydroxypropyl)trimethylammonium formate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification: Hydroxypropyl, 2-, trimethylammonium formate
Synonym: DABCO TMR-2® Catalyst
Sponsor physical state/Appearance: Clear colorless liquid
Envigo physical state/Appearance: Colorless to very light blue liquid
CAS Number: 62314-25-4
Batch: Chernil.20160628.B
Purity: 95.25% active in water
Expiry Date: 28 June 2017
Storage Conditions: Room temperature, in the dark, stored over silica gel
from 08 November 2016

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the control and 100 mg/L test vessel at 0 and 72 hours from fresh media and at 24 and 96 hours from old media for quantitative analysis. The samples were stored frozen prior to analysis.
Duplicate samples and samples at 24 (fresh media), 48 (old and fresh media) and 72 hours (old media) were taken and stored frozen for further analysis if necessary.

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The test was carried out using juvenile rainbow trout (Oncorhynchus mykiss). Fish were obtained from Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK and maintained in-house. Fish were maintained in a glass fiber tank with a "single pass" water renewal system. Fish were acclimatized to test conditions from 12 June 2017 to 19 June 2017. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.

The water temperature was controlled at approximately 14 °C with a dissolved oxygen content of greater than or equal to 9.4 mg O2/L. These parameters were recorded daily. The stock fish were fed commercial trout pellets which was discontinued approximately 24 hours prior to the start of the definitive test.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

Seven fish were exposed to an aqueous solution of the test item, at a single concentration of 100 mg/L for a period of 96 hours at a temperature of approximately 15 ºC to 16 °C under semi-static test conditions.

Post exposure observation period:

No

Hardness:

total hardness approximately 140 mg/L as CaCO3

Test temperature:

The water temperature was controlled at approximately 14 °C

pH:

7.7-8.0

Dissolved oxygen:

dissolved oxygen content of greater than or equal to 9.4 mg O2/L.

Salinity:

N/A

Nominal and measured concentrations:

Analysis of the fresh test preparations at 0 and 72 hours and old test solutions at 24 and 96 hours showed measured test concentrations to be near nominal and so the results are based on the nominal test concentration only.

Details on test conditions:

Seven fish were exposed to an aqueous solution of the test item, at a single concentration of 100 mg/L for a period of 96 hours at a temperature of approximately 15 ºC to 16 °C under semi-static test conditions.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Exposure of rainbow trout (Oncorhynchus mykiss) to the test item gave LC50 values based on the nominal concentration of greater than 100 mg/L.
There were no mortalities in 7 fish exposed to a test concentration of 100 mg/L for a period of 96 hours. There were no sub-lethal effects of exposure observed in the test.

The No Observed Effect Concentration was 100 mg/L.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.

Reported statistics and error estimates:

An estimate of the LC50 values was given by inspection of the mortality data.

Validity criteria fulfilled:

yes

Conclusions:

The test was considered to be valid given that none of the control fish died or showed signs of stress during the test and that the oxygen concentration at the end of the test was equal to or greater than 60% of ASV (6.1 mg O2/L at 15 °C) in the control and test vessels.

Exposure of rainbow trout (Oncorhynchus mykiss) to the test item gave LC50 values based on the nominal concentration of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.

Description of key information

Exposure of rainbow trout (Oncorhynchus mykiss) to the test item gave LC50 values based on the nominal concentration of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L