Trisodium 5-[[4-chloro-6-(methylphenylamino)-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that the source and the target substance have very similar physicochemical and (eco)toxicological properties because their chemical structures are nearly identical. An analogue approach has thus been employed. The target substance is Reactive Red 24 carrying a methyl group, while the source chemical is Reactive Red 24:1 carrying an ethyl group.
The presence of sulphonate groups make both dyes highly water soluble and therefore less critical for human health and environmental issues. Based on their chemical similarity, similar properties are expected in both humans and the environment.
2. SOURCE AND TARGET CHEMICAL(S)
Source: Reactive Red 24:1 (CAS# 72829-25-5 / EC# 276-911-4)
Target: Reactive Red 24 (CAS# 70210-20-7 / EC# 274-417-3)
3. ANALOGUE APPROACH JUSTIFICATION
see attachment under 4.12 Auto flammability

Reason / purpose for cross-reference:

read-across source

Parameter:

other: biodegradation measured as BOD vs ThOD

Value:

12

Sampling time:

28 d

Parameter:

BOD5

Value:

100 mg O2/g test mat.

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Biodegradation of the substance after 28 days was 12%. The substance is not readily nor inherently biodegradable.

Executive summary:

A key study was performed to determine the percent biodegradation of the source substance. The inherent biodegradability was determined in a 28 days MITI (II) - test according to OECD TG 302C. The source substance was tested in a concentration of 30 mg/l. The reference substance (Sodium benzoate) was tested in a concentration of 100 mg/l. Based on the results of the study, biodegradation of the test substance after 28 days was 12 %. The structurally related target substance will show similar behaviour.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

BOD value

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

None

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

5 d

Details on study design:

None

Preliminary study:

None

Test performance:

None

Details on results:

None

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

None

None

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The BOD of FAT 40034 is: 0.0 mg O2/g test mat.

Executive summary:

The biochemical oxygen demand of FAT 40034 is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. Based on the findings from the study, the BOD5 of FAT 40034 is 0.0 mg O2/g test mat.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

COD value

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

None

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

5 d

Details on study design:

None

Preliminary study:

None

Test performance:

None

Details on results:

None

Key result

Parameter:

COD

Value:

798 mg O2/g test mat.

Results with reference substance:

None

None

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The COD of the test substance FAT 40034 is: 798 mg 02/g.

Executive summary:

A study was performed to determine the chemical oxygen demand of FAT 40034. Based on the findings from the study, the COD of FAT 40034 is 798 mg02/g

Reference 4

Endpoint:

biodegradation in water: inherent biodegradability

Remarks:

OECD 302C

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Deviations:

yes

Remarks:

The test substance was running in duplicate instead in triplicate.

Principles of method if other than guideline:

None

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Test medium:
3 ml of each following stock solutions were added per 1 litre distilled water to form the test medium:

Stock solution A:
8,50 g of potassium acid phosphate, 21,75 g of di-potassium hydrogen phosphate, 22,18 g of di-sodium hydrogenphosphate dihydrate and 1,70 g of ammonium chloride were dissolved in distilled water and adjusted to 1 litre. The pH value of this solution was 7,2.

Stock solution B: 22,50 g of magnesium sulfate heptahydrate were dissolved in distilled water and adjusted to 1 litre.
Stock solution C: 36,40 g of calcium chloride dihydrate were dissolved in distilled water and adjusted to 1 litre.
Stock solution D: 0,25 g of ferric chloride hexahydrate were dissolved in distilled water and adjusted to 1 litre.

Oxygen conditions:

aerobic

Inoculum or test system:

other: Mixture of polyvalent bacteria collected on April 23, 1996 in the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal.

Details on inoculum:

Mixture of polyvalent bacteria collected on April 23, 1996 in the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal. Bacteria concentration: 100 mg/l suspended solids.

Duration of test (contact time):

28 d

Details on study design:

Performance of the test:
This test method was based on the following conditions:
- test chemicals as sole organic carbon sources
- no adaptation of micro-organisms to the test chemicals
Tested chemicals were inoculated in the testing vessels with micro-organisms. During the test period, the Biochemical Oxygen Demand was measured continuously by means of a BOD-meter. Biodegradability was calculated on the basis of the theoretical oxygen demand (ThOD) or less satisfactorily COD, if the ThOD could not be calculated. Optionally, primary biodegradation could be determined by analysis of dissolved organic carbon (DOC) at the beginning and the end of the test.

Reference substance:

benzoic acid, sodium salt

Preliminary study:

None

Key result

Parameter:

other: Biodegradation measured as BOD versus ThOD

Value:

12

Sampling time:

28 d

Remarks on result:

other: Standard deviation is not available

Details on results:

Test substance:
The biodegradation of FAT 40034/D corrected by the inoculum control and measured as
BOD versus ThOD was: 12% after 28 days
The BOD5 after 5 days was: 100 mg O2/g

Abiotic control:
The abiotic degradation at the end of the test and measured as BOD versus ThOD was: 0 %

Parameter:

BOD5

Value:

100 mg O2/g test mat.

Results with reference substance:

The biodegradation of the reference substance corrected by the inoculum control and measured as BOD versus ThOD was 84 % after 7 days
and was: 94 % after 14 days
and was: 100% after 28 days

None

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Biodegradation of the test substance after 28 days was 12 %.

Executive summary:

A key study was performed to determine the percent biodegradation of FAT 40034. An inherent biodegradability of FAT 40034/D was determined in a 28 days MITI (II) - Test according to the OECD Guideline for Testing of Chemicals, No. 302C. The test substance was tested in a concentration of 30 mg/l. The reference substance (Sodium benzoate) was tested in a concentration of 100 mg/l. Based on the results of the study, biodegradation of the test substance after 28 days was 12 %.

Description of key information

The substance is a reactive dye and as such the substance should not be considered to be biodegradable at all.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The target substance is a reactive dye and as such the substance should not be considered to be biodegradable at all. The key study was performed according to OECD TG 302B, inherent biodegradation of the source substance after 28 d was 12%, confirming that the substance should not be considered as inherently or readily biodegradable under the conditions of this test. In addition determination of BOD and COD values and calculation of the BOD5/COD quotient resulted in a value of zero. Based on this the read-across source substance is regarded to be not biodegradable at all. Therefore, the target substance is also considered to be not biodegradable what is expected for a reactive dye.