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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Remarks:
HPLC analysis and UV/VIS-detection
Details on sampling:
Sampling schedule:
- Control: at 48 hours (on 2017-12-07)
- Test concentration/s: 0.40, 0.88, 1.9, 4.3, 9.4, 20.7, 45.45 and 100 mg/L in the freshly prepared media (on 2017-12-05 and 2017-12-06) and in the media after 24 h of exposure (on 2017-12-06 and 2017-12-07) according to the semi-static conditions
Vehicle:
no
Remarks:
Auxiliaries: ultra turrax and magnet stirrer
Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnia magna STRAUS, parthenogenetic females
- Source: Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure has been maintained for more than 20 years in the test facility under constant temperature conditions (20 +/-2 °C) at a 16 : 8 hour light-dark photoperiod (light intensity: < 20 µE x m-2 x s-1). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test
- Culture and dilution water: Reconstituted water (so-called 'M4 medium' according to OECD 202 and EC Method C.2, annex 1)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
14.6 °dH (= 261 mg/L CaCO3)
Test temperature:
19.6 - 20.6 °C
pH:
7.2 - 8.1
Dissolved oxygen:
8.2 - 8.8 mg/L
Salinity:
n.a.
Conductivity:
No data
Nominal and measured concentrations:
Analysis (HPLC) of test substance after 24 hours of exposure:
Nominal test concentrations 0.40, 0.88, 1.9, 4.3, 9.4, 20.7, 45.45 and 100 mg/L equal 0.152, 0.334, 0.716, 1.607, 3.535, 7.292 and 35.436 mg/L (HPLC)

Effective concentrations ranged from 94.3 % to 103.8 % in the freshly prepared media and from 93.0 % to 103.6 % in the media after 24 hours of exposure.
Details on test conditions:
Exposure conditions
- Test vessels: 100 mL glass beakers covered with watch glasses holding 5 neonates in 50 mL of test medium
- Experimental design: 8 test concentrations plus 1 control; 5 neonates per vessel, 4 replicates per concentration/control;
- Feeding: none during the exposure period
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 16 h light : 8 h dark
- Temperature of incubation unit: 19.6 to 20.0 °C
- Aeration: none
- Test item concentration/s: 0.40, 0.88, 1.9, 4.3, 9.4, 20.7, 45.45 and 100 mg/L
- Method of administration: stock solution (100 mg/L)
- Medium renewal: daily
- Criteria of effects: item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The results are expressed in terms of nominal concentrations as sum of Reaction mass of methyl 2-methylbenzene-1-sulfonate and methyl toluene-4-sulfonate, o-toluenesulfonic acid and p-toluenesulfonic acid. Effective concentrations ranged from 94.3 % to 103.8 % in the freshly prepared media and from 93.0 % to 103.6 % in the media after 24 hours of exposure.
The EC 50 was calculated by probit analysis using the statistics programme ToxRatPro Version 2.10 (released 2010-09-10).
Validity criteria fulfilled:
yes
Remarks:
Immobilisation and other abnormalities in the controls did not exceed 10 % by the end of the test; dissolved oxygen concentration remained above 3 mg/L throughout the exposure period
Conclusions:
After 48 hours an EC50 of 7.5 mg/L was determined for test substance towards Daphnia under exposure conditions. The results are expressed in terms of nominal concentrations as sum of Reaction mass of methyl 2-methylbenzene-1-sulfonate and methyl toluene-4-sulfonate, o-toluenesulfonic acid and p-toluenesulfonic acid. Effective concentrations ranged from 94.3 % to 103.8 % in the freshly prepared media and from 93.0 % to 103.6 % in the media after 24 hours of exposure.
Executive summary:

A study was performed to assess the acute toxicity of the test substance to Daphnia magna STRAUS under semi-static conditions.

It was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004).

A range finding test preceded the main test. It provided information about the range of concentrations which were used in the main test. The following nominal concentrations of the test item were tested in the range finding test: 0.1, 1, 10 and 100 mg/L.

The Daphnia were exposed to a range of concentrations, nominally 0.40, 0.88, 1.9, 4.3, 9.4, 20.7, 45.45 and 100 mg/L of the test substance in dilution water. Auxiliaries used to prepare the test media were an ultra turrax and a magnetic stirrer. 20 animals were exposed per concentration/control in 4 vessels a 5 neonates. The test was conducted as a semi-static test with daily renewal of test medium. During the test a temperature range of 18 - 22 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in each individual test. The temperature, the pH and the oxygen values were measured every day in the freshly prepared media and in the media after 24 hours of exposure.

Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The EC 50 was calculated by probit analysis using the statistics programme ToxRatPro Version 2.10 (released 2010-09-10). An 48h-EC50 of 7.5 mg/L was determined.

The results are expressed in terms of nominal concentrations as sum of Reaction mass of methyl 2-methylbenzene-1-sulfonate and methyl toluene-4-sulfonate, o-toluenesulfonic acid and p-toluenesulfonic acid. Effective concentrations ranged from 94.3 % to 103.8 % in the freshly prepared media and from 93.0 % to 103.6 % in the media after 24 hours of exposure.

Description of key information

A study was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004) to assess the acute toxicity of the test substance to Daphnia magna STRAUS.

The Daphnia were exposed to a range of concentrations, nominally 0.40, 0.88, 1.9, 4.3, 9.4, 20.7, 45.45 and 100 mg/L of the test substance in dilution water. 20 animals were exposed per concentration/control in 4 vessels a 5 neonates. The test was conducted semi-static with daily renewal of test medium.

Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. An 48h-EC50 of 7.5 mg/L was determined.

The results are expressed in terms of nominal concentrations as sum of Reaction mass of methyl 2-methylbenzene-1-sulfonate and methyl toluene-4-sulfonate, o-toluenesulfonic acid and p-toluenesulfonic acid. Effective concentrations ranged from 94.3 % to 103.8 % in the freshly prepared media and from 93.0 % to 103.6 % in the media after 24 hours of exposure.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
7.5 mg/L

Additional information