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EC number: 921-114-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Sep 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure and at the renewal (24 and 48 h), samples of the freshly prepared test item concentrations were taken and analyzed.
At renewal and at the end of the exposure (24 and 48 h), sampled of the 24 h old media were taken directly fron the test vessels and analyzed. - Vehicle:
- yes
- Remarks:
- dilution water
- Details on test solutions:
- Stock solution:
A stock solution (100 mg/L of the test item were weighed out) was freshly prepared with dilution water prior to the start of the exposure (0 h) and prior to the renewal of the test solutions (24 h). The stock solution was mixed thoroughly by manual agitation.
Test concentrations:
5 test item concentrations ina geometirc series with a separation factor of 2, prepared by dilution of the stock solution of 100 mg/L with dilution water were tested as follows: 6.25, 12.5, 25.0, 50.0 and 100 mg/L.
Control:
Dilution water without test item incubated under the same semi-static conditions as the test group. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden und Lufthygiene (WaBoLu), Berlin, Germany
- Age: < 24 h
FEEDING DURING TEST
- Food type: 5 x per week ad libitum with a mix of unicellular green algae - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 0 h: 170 mg CaCO3/L
24 h: 178 mg CaCO3/L - Test temperature:
- 20.8 - 20.9 °C
- pH:
- 0 h: 8.61
24 h: 8.24 - Dissolved oxygen:
- 0 h: 8.76 mg/L
24 h: 8.90 mg/L - Conductivity:
- 0 h: 448 µS/cm
24 h: 462 µS/cm - Details on test conditions:
- Test vessels:
Glass beakers, 50 mL capacity, loosely covered with watch glasses
Test volume:
20 mL
Number of daphnids and replicates:
20 daphnids, divided into 4 replicates, each with 5 daphnids were used for each concentration level and control.
Application:
20 g test solution per replicate was weighed out into each test vessel. This corresponds to 20 mL per test vessel. The daphnids were inserted with a samll amount of dilution water (start of the exposure) or test solution (water renewal) by pipette.
Renewal of the test solutions:
The test solutions were renewed after 24 h. For this purpose, a second set of test vessels was filled with the freshly prepared test solutions and the daphnids were transferred by pipette. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item, the 48 h EC50 for Daphnia magna was 10.8 mg/L (95% confidence limits: 6.48 - 12.4 mg/L).
- Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202 (2004).
The study was conducted under semi-static conditions over a period of 48 hours with 5 concentrations of the test item in the range of 6.25 to 100 mg/L, prepared in a geometric series with a separation factor of 2.
All tested concentration levels were visually clear throughout the exposure. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control.
The concentrations of the test item were analytically verified via HPLC-DAD in fresh media of all concentration levels and in the control at the start of the exposure and at renewal (0 and 24 hours) and in 24-hours old media at renewal and at the end of the test (24 and 48 hours).
The measured concentrations of the test item in fresh media at the start of the exposure and at renewal (0 and 24 hours) were in the range of 90 to 100% of the nominal values, and 88 to 100% in old media at renewal and at the end of the test (24 and 48 hours).
The measured test item concentrations were all within ± 20% of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, all effect concentrations are based on the nominal concentrations of the test item.
The validity criteria of the test guidelines were fulfilled.
Based on the nominal concentrations of the test item, the 48 hour-EC50 for Daphnia magna was 10.8 mg/L (95% confidence limits: 6.48 - 12.4 mg/L).
Reference
Table 1: Immobilization rates after 24 and 48 h
Nominal test item conc. [mg/L] | Immobilization [%] | |||||||||
24 h | 48 h | |||||||||
Replicates | Replicates | |||||||||
1 | 2 | 3 | 4 | MV | 1 | 2 | 3 | 4 | MV | |
100 | 100 | 100 | 100 | 100 | 100 | 100 % mortality after 24 h | ||||
50.0 | 100 | 100 | 100 | 100 | 100 | 100 % mortality after 24 h | ||||
25.0 | 80 | 100 | 100 | 100 | 95 | 100 | 100 | 100 | 100 | 100 |
12.5 | 20 | 0 | 20 | 20 | 15 | 60 | 80 | 80 | 100 | 80 |
6.25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Description of key information
OECD 202: 48 -h EC50 = 10.8 mg/L (nominal)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 10.8 mg/L
Additional information
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202 (2004).
The study was conducted under semi-static conditions over a period of 48 hours with 5 concentrations of the test item in the range of 6.25 to 100 mg/L, prepared in a geometric series with a separation factor of 2.
All tested concentration levels were visually clear throughout the exposure. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control.
The concentrations of the test item were analytically verified via HPLC-DAD in fresh media of all concentration levels and in the control at the start of the exposure and at renewal (0 and 24 hours) and in 24-hours old media at renewal and at the end of the test (24 and 48 hours).
The measured concentrations of the test item in fresh media at the start of the exposure and at renewal (0 and 24 hours) were in the range of 90 to 100% of the nominal values, and 88 to 100% in old media at renewal and at the end of the test (24 and 48 hours).
The measured test item concentrations were all within ± 20% of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, all effect concentrations are based on the nominal concentrations of the test item.
The validity criteria of the test guidelines were fulfilled.
Based on the nominal concentrations of the test item, the 48 hour-EC50 for Daphnia magna was 10.8 mg/L (95% confidence limits: 6.48 - 12.4 mg/L).
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