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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Name and location of sewage treatment plant where inoculum was collected: Stellinger Moor, Hamburg, Germany
- Preparation of inoculum for exposure: 50 mL synthetic wastewater added to 1 L sludge. Aerated over night.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Nominal and measured concentrations:
200, 400, 800 mg/L + control
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks:
- Sludge concentration (weight of dry solids per volume): 2.2 g/L dry weight

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sewage from Stadtwerke Norderstedt
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
525 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
3 h
Dose descriptor:
EC10
Remarks:
estimated from graph
Effect conc.:
250 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: EC50 15 mg/L 3,5-Dichlorophenol
Validity criteria fulfilled:
yes
Conclusions:
In a test on sludge respiration inhibition according toOECD 209, the EC50 was determined to be 525 mg/L after 3 h.

Description of key information

In a test on sludge respiration inhibition according to OECD 209, the EC50 was determined to be 525 mg/L after 3 h.

Key value for chemical safety assessment

EC50 for microorganisms:
525 mg/L
EC10 or NOEC for microorganisms:
250 mg/L

Additional information