3-(diethylamino)-7-imino-7H-[1]benzopyrano[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 35.2 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

2.Based on the immobilisation of daphnia magna due to the exposure of chemical for 48 hrs, the median effective concentration EC50 was determine to be 10.9 mg/l.

3.After the incubation period of 48 hrs of test organism with the test chemical, effect were observed at 11 mg/l.

Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic as the value ranges from 10.9 -35.2 mg/l and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Toxicity to aquatic algae:

1. Based on the growth rate inhibition of Desmodesmus subspicatus, by the test chemical after the exposure for 72 hrs, the ErC50 value was determine to be 17.5 mg/l.

2. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L on the basis of effects on growth rate in a 72 hour study.

Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic as value ranges from 13.4 -17.5 mg/l and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Additional information

Summarized result for the determination of nature of chemical 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1) on the growth and other biological and physical activity of aquatic invertebrates, algae and cyanobacteria when chemical comes in contact with test organisms, by considering the data for structurally and functionally similar read across chemicals from various sources which are as follows:   

 

Short term toxicity to aquatic invertebrates:

Short term toxicity of 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/L was prepared by dissolving clear yellow oily liquid in DMSO. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted at various different nominal concentrations 0, 0, 4, 10, 17, 30, 55 and 100 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 35.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrates and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

In the second study for another read across chemical toxicity was measured. Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving white powder in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Acetone used as a solvent because of limited solubility of tested sample in water. 0, 0, 0.5, 1, 2, 4, 8, 16 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be10.9 mg/L with the confidence interval of 9.9 - 11.9 mg/l on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Similarly short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals. Study was conducted to determine the nature and effects of test chemical on the growth of aquatic invertebrate. Study was conducted according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under the static system by providing the exposure period of 48 hrs. After the incubation period of 48 hrs of test organism with the test chemical, effect were observed at 11 mg/l. based on the EC50, chemical was consider as toxic and can be consider to be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole -6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

Toxicity to aquatic algae:

Short term toxicity of 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1) on the growth and other biological activity of aquatic algae is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the growth of aquatic algae and cyanobacteria. Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The stock solution 100 mg/l was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and performed at various nominal concentrations 0, 0, 6, 12, 24 and 100 mg/L. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 17.5 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the ErC50 value, it can be concluded that the substance is likely to be hazardous to aquatic algae and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

Similarly short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals. Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The stock solution (100 mg/L) was prepared by dissolving pinkish red powder in OECD growth medium. The stock solution was kept in ultrasonic bath for 20min Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and performed at various nominal concentrations 0, 2.5, 5, 10, 20 and 40 mg/L. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 13.4 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic algae and cyanobacteria and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole -6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

  

Hence on the basis of all the above studies for toxicity to aquatic invertebrates and algae, it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.