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EC number: 700-881-8 | CAS number: 83145-78-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-09-20 to 2004-10-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- , directive 92/69, publication no. L383, December 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- , adopted 1984-04-04
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 244761-29-3
- EC Number:
- 607-383-9
- Cas Number:
- 244761-29-3
- IUPAC Name:
- 244761-29-3
- Reference substance name:
- Lithium bis(oxalato)borate
- EC Number:
- 456-990-3
- EC Name:
- Lithium bis(oxalato)borate
- IUPAC Name:
- lithium bis(oxalato)borate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- SMILES: O=C1O[B-]2(OC1=O)OC(=O)C(=O)O2.[Li+]
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling:
Frequency: 1 . At the start of the test and after 24 hours from the freshly prepared solutions. 2. At the first renewal (t=24h) and the end of the test from the 24-hour old solutions.
Volume: 20 ml from the approximate centre of the test vessel.
Storage: Samples were stored at room temperature.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Preparation of test solutions started with a stock solution of 100 mg/L. No special treatment other than a short period of mixing was necessary to completely dissolve this stock solution in test medium. The stock was clear and colourless. However, due to the extremely instable character of the test substance the solution became increasingly hazy within a short time with pH-values dropping to acidic Ieveis. The pH of the stock solutions was consequently re-adjusted to approximately 8.5 using sodium hydroxide. The hazy suspension was then filtered through a rough paper filter (Schleicher & Schuell 604) to remove the major part of undissolved test particles (> ca. 5 µm). The clear and colourless filtrate was then used for testing. The lower test concentrations for the range-finding test were prepared by subsequent dilution of the filtrate in test medium. Alltest solutions were renewed after 24 hours of exposure. Note that the time for preparation of the stock and test solutions was kept as short as possible with a maximum preparation time of approximately 30 minutes.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: ln-house Iabaratory culture with a known history
- Max. age at study initiation: 4 weeks
- Feeding during test: No
- Food type: suspension of fresh water algae
- Frequency: daily
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Water hardness: 250 mg CaCO3 / L
- Test temperature:
- start: 20.3 °C
During the test: 19.2 - 20.0 °C - pH:
- 7.8 - 8.5
- Dissolved oxygen:
- 8.4 - 9.0
- Nominal and measured concentrations:
- A filtered solution prepared at a loading rate of 100 mg/L was used for the main test. The concentration Lithium present in the samples was taken as a
measure for the Lithium bis(oxalato)borate concentration present in the test solutions. Analysis of the samples taken during the Iimit test showed that measured Lithium concentrations were stable between 3.4 and 3.6 mg/L during the 24-hour refreshment periods and in agreement with what was expected based on the theoretical percentage Lithium (3.58 %) in the test substance. All results will however be based on the loading rate as measurement of Lithium can only be considered indicative for the real Lithium bis(oxalato)borate concentration present in the test solution. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Fill volume: 80 ml
- Aeration: No
- Renewal rate of test solution: semi-static with renewal of test solutions after 24 hours
- No. of organisms per vessel: 5 per vessel
- No. of vessels per concentration or vehicle control (replicates): 4 (20 daphnia per concentration)
- Medium: ISO (M7)
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16 hours daily
TEST CONCENTRATIONS
- Range-finding test: 2x5 daphnia per contentration were exposed to vehicle control, a filtrate prepared at a loading of 100 mg/L and dilutions of the filtrate containing 0.1, 1.0 and 10 % of the filtrate. No immobilisation was observed during 48-hours exposure.
- Test concentrations: Therefore, in the main test a limit test with a filtrate prepared at a loading of 100 mg/L was performed. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: A filtered solution prepared at a loading rate of 100 mg/L
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: A filtered solution prepared at a loading rate of 100 mg/L
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: A filtered solution prepared at a loading rate of 100 mg/L
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: A filtered solution prepared at a loading rate of 100 mg/L
- Basis for effect:
- mobility
- Details on results:
- No immobilisation was observed.
Any other information on results incl. tables
Read across for evaluation of short term toxicity to aquatic invertebratesof potassium bis(oxalato)borate from the toxicity study results of lithium bis(oxalato)borate is justified on the basis of structural similarity of the substances. The bis(oxalato)borate salt part [B(C2O4)2] is the same in both substances and only the cationic part of salts differ (lithium or potassium). Both lithium and potassium belong to alkali-metals.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Lithium bis(oxalato)borate did not reveal acute toxicity to Daphnia magna in a semi-static test resulting in a 48h EC50 of above 100 mg/L. The corresponding NOEC is 100 mg/L, respectively. Based on structural similarity, the result can be used for read across when evaluating acuatic toxicity of potassium bis(oxalato)borate. The study result is valid for environmental classification of both substances (LiBOB and KBOB). For risk assessment, also potassium bis(oxalato)borate can be regarded as not acutely toxic to Daphnia mgna. See also attachment in section 13 (Analogue approach justification).
- Executive summary:
Lithium bis(oxalato)borate was assessed in a short term toxicity study to Daphnia magna according to OECD 202 and EU method C.2.The test substance was completely soluble in test medium at the concentrations tested. The entire project was performed applying semi-static test conditions, with renewal of test solutions after 24 hours of exposure because the test substance was known to be highly unstable in water. Test solutions were prepared starting with a stock solution at 100 mg/L. The initial test solution was clear and colourless but, almost instantaneous after dissolving the test substance in the test medium, the solution turned increasingly hazy and the pH dropped to acidic levels. The pH was adjusted to approximately 8.5 as a consequence of the observed steep pH fall.
The project started with a range-finding test exposing ten daphnia per concentration (in duplicate, 5 per vessel) to a blank-control, a test substance filtrate prepared at a loading rate of 100 mg/L and dilutions of the filtrate containing 0.1, 1.0 and 10 % of the filtrate. No immobile daphnia were observed during the 48-hour exposure period in the undiluted filtrate or any of the filtrate dilutions tested. Hence, the expected 48h EC50 exceeded the concentration present in a filtered solution prepared at a loading rate of 100 mg/L. The project was continued with a limit test exposing twenty daphnia per concentration (in quadruplicate, 5 per vessel) to a blank-control and a filtered test substance solution prepared at a loading rate of 100 mg/L.
The total test period was 48 hours. Samples for analytical confirmation of actual exposure concentrations were taken from the freshly prepared solutions at the start and after 24 hours of exposure and at the end of the test period. Analysis of the samples taken during the limit test showed that measured lithium concentrations were stable between 3.4 and 3.6 mg/L during the 24-hour refreshment periods and in agreement with what was expected based on the theoretical percentage of lithium (3.58 %) in the test substance. All results will however be based on the loading rate as measurement of lithium can only be considered indicative for the real test substance concentration present in the test solution.
The study met the acceptability criteria prescribed by the protocol and was considered valid. The test substance did not induce acute immobilisation of Daphnia magna at a loading rate of 100 mg/L after 48 hours of exposure (NOEC). The 48h EC50 exceeded a loading rate of 100 mg/L, the regulatory limit concentration. (NOTOX, 2004)
Based on structural similarity, results from the acute acuatic toxicity study can be used for read across when evaluating aquatic toxicity of potassium bis(oxalato)borate. Consequently, also potassium bis(oxalato)borate can be regarded as not acutely toxic to Daphnia magna.
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