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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
December 1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
OECD 203

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,6-dichlorobenzyl chloride
EC Number:
217-940-4
EC Name:
2,6-dichlorobenzyl chloride
Cas Number:
2014-83-7
Molecular formula:
C7H5Cl3
IUPAC Name:
2,6-dichlorobenzyl chloride

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Remarks:
0.1 ml Aceton

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Hamilton-Buchanan

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21.6 - 23°C
pH:
7.1 - 8.4
Dissolved oxygen:
5.8 - 8.8 mg/L
Nominal and measured concentrations:
0 mg/L
0 mg/L with 0.1 ml Aceton
1 mg/L
1.25 mg/L
1.8 mg/L
2.5 mg/L
3.5 mg/L
10 mg/L

Substance deposit was observed for 1 / 2,5 / 3,5 / 10 mg/L. Concentrations refer to total amount in aquaria not the soluted amount.
Details on test conditions:
- number of fish/vessel: 10
- test vessel: 10-l aquaria
- static system
- observations: death, behaviour

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 1 - <= 1.25 mg/L
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1 mg/L
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1.8 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
>= 1.25 - <= 1.8 mg/L
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
1 mg/L
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
1.8 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50 of 2,6-Dichlorobenzyl chloride is between 1 and 1,25 ml/L within 96 h for Danio rerio.
Executive summary:

The acute toxicity for the test item 2,6-dichlorbenzoyl chloride to zebrafish (Brachydanio rerio) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1984), EC Commission Directive 84/449/EC Method C.1 (1984) and according to German Chemikaliengesetzt (Texte 16/48 Umweltbundesamt). Testing laboratory was Deptm. Pharma Forschung Toxikologie u. Pathologie at Hoechst AG Frankfurt/Main, Germany. A static test was conducted. Duration of the test was 96 h. 10 test organisms were exposed to 0, 1, 1.25, 2.5, 3.5 and 10 mg/L of 2,6-dichlorbenzoyl chloride, additionally to the solvent control containing 0.1 ml acetone. Water quality parameters (pH, temperature, oxygen content) were determined at time points 0, 3, 24, 72, and 96 hours to be within the acceptable limits. No lethality occurred in the control groups.

At 1 mg/L symptoms were observed but no letality. At 1.25 - 10 mg/L symptoms and lethality were observed.