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EC number: 216-630-6 | CAS number: 1631-25-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 July to 22 December 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The temperature in the algae incubator dropped 0.4°C below the allowed range but had no effect on the outcome or intergrity of the result.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Nippon Shokubai Co. Ltd., Kogin Building, 4-1-1 Koraibashi, Chuo-ku, Osaka 541-0043, Japan. Lot/batch number: 5K21
- Expiration date of the lot/batch: 26 May 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient (15 +/- 10°C). Protected from light, 40°C or lower (SDS)
- Stability in container after opening: Stable
- Solubility and stability of the test substance in the solvent/vehicle: Stability in water: - Undergoes Hydrolysis
OTHER SPECIFICS:
- Appearance (specified): Solid White Flake
- Appearance (observed): White Solid Flakes
- Solubility (specified): 0.3g/L (0.03g/100ml)
- 0.03g/100g water at 25°C (SDS)
- Homogeneity (specified): Homogeneous
- Homogeneity (observed): Homogeneous flakes of various sizes - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Dilution water: The stocks of algal culture were maintained, and the tests performed, in nutrient growth medium (OECD 201) which was prepared by adding appropriate amounts of nutrient stocks to deionised water (sterilised by autoclaving at 120°C for 15 minutes) at a pH of 8.30 (adjusted to 8.20) (See 2.5 Test methods & conditions).
- Preparation of test solutions: Information provided by the Sponsor indicated that the sample was soluble at 0.3g/L (0.03g/100ml), 0.03g/100g water at 25°C (SDS). Stock solutions were prepared for both the range-finding and definitive tests by addition of the test sample directly to nutrient growth medium in a volumetric flask of adequate volume. Calculated volumes of the stock solution were added to nutrient growth medium and made to volume to prepare the test concentrations and provide enough solution for testing and subsequent water quality measurements. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland, PA37 1QA
- Pre-culture conditions (prior to the test start):
- Temperature: 21.1 – 21.5ºC
- Illumination: 6440 - 7930 lux continuous white light
- Orbiting: set to 200rpm
- Culture media: Deionised water with added nutrients according to OECD 201. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ± 2 hours
- Test temperature:
- Temperature range within incubator throughout test: 20.6 - 21.5°C
- pH:
- Initial pH at 0 Hours: 8.20
pH range in control and test concentrations throughout test: 7.45 - 8.20 - Nominal and measured concentrations:
- Nominal; 0 (control), 0.1, 0.32, 1.0, 3.2, 10 and 32mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flask
- Material, size, headspace, fill volume: glass, 250 mL, 100 mL test volume
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): None
- Initial cells density: 1xE04
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterilised deionised water with added nutrients according to OECD 201.
- Intervals of water quality measurement: The temperature (to 0.1ºC) and light intensity (lux) within the incubator was recorded at the beginning of the study, after 24, 48 hours and at the end of the 72 hour test period. The pH (to 0.01) and temperature (to 0.1°C) were recorded for each test and control solution at the beginning of the test and on the pooled replicates at the end of the 72-hour test period.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 72h
- Light intensity and quality: 6000-7910 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cell densities were measured microscopically by direct cell counts on each test and control replicate. Six counts were taken of each control replicate at 24 hours and 3 counts were taken of each control replicate at 48 and 72 hours (±2h). 3 counts were taken for each test concentration replicate at 24, 48 and 72 hours (±2h). The cell counts were made using a haemocytometer and microscope.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 0.3
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.646 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% Confidence Limits
- Remarks:
- (0.471 - 0.884mg/l)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- determined by Bonferroni t Test
- Details on results:
- Test observations:
- All cells within the control and all test concentrations appeared normal, no morphological abnormalities were observed.
- When observed visually, the control and all test conical flasks appeared colourless after 24 hours.
- At 48 hours the control and 0.1mg/l appeared slightly green with all other concentrations clear and colourless.
- At 72 hours, the control and 0.1mg/l appeared pale green, 0.32 and 1.0mg/l appeared slightly green and 3.2, 10 and 32mg/l were clear and colourless. The lack of green colouration in the 3.2, 10 and 32mg/l test concentrations indicates a lack of algal growth throughout the 72 hour test period. - Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 was found to be 0.646 mg/L. This is also regarded as a NOEC and gives a classifciation of Aquatic chronic 3.
Reference
Description of key information
The ErC50 was found to be 0.646 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.646 mg/L
- EC10 or NOEC for freshwater algae:
- 0.646 mg/L
Additional information
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