Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
23 Feb - 14 Mar 1986
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report Date:
1986

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Test Methode relating to New Chemical Substances (Kanpogyo N° 5, Yakuhatsu N° 616, 49 Kikyoku N° 392)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Conventional activated sludge purchased from Chemical Safety Center, Chemicals Inspection & Testing Inst., Japan on February 20, 1986
- Storage conditions: Conventional activated sludge was cultured in the culture system in the draft under the conditions of temperature 25 ± 2°C, pH 7.0 ± 1.0, and ventilation volume ≥5 ppm.
- Storage length: Cultured for 4 days
- Concentration of sludge: The activated sludge concentration was measured using a sludge densitometer (CENTRAL KAGAKU CORP: ML-52, SOP /INS /4.28).

Duration of test (contact time):
14 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus manufactured by Ohkura Electric Co., Ltd.
- Measuring equipment: Closed system oxygen consumption measuring apparatus manufactured by Ohkura Electric Co., Ltd. Coulometer: Identification No.1 (SOP/INS/4.5)

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
8.3
Sampling time:
14 d
Remarks on result:
other: Mean value; BOD degradation
Parameter:
% degradation (O2 consumption)
Value:
10.5
Sampling time:
14 d
Remarks on result:
other: Mean value; direct determination based on HPLC

BOD5 / COD results

Results with reference substance:
Because the degradability of aniline was 66.0% after 7 days, good degradability was confirmed.

Applicant's summary and conclusion

Interpretation of results:
not readily biodegradable
Conclusions:
About 90% of the test item is not completely mineralized, and the test item is a persistent chemical substance under the conditions specified in the Law Concerning the Examination and Regulation of Manufacture, etc. of Chemical Substances.