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Diss Factsheets
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EC number: 208-719-3 | CAS number: 539-48-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch No.: not specific
Purity: not specific - Analytical monitoring:
- no
- Details on sampling:
- The test item be directly dispersed in culture medium. Samples were taken at 0, 24, 48 and 72hours and the absorbance measured at 665nm using a Jenway 6100 Spectrophotometer.
- Vehicle:
- no
- Details on test solutions:
- The test item be directly dispersed in culture medium.
- Test organisms (species):
- Scenedesmus sp.
- Test type:
- static
- Water media type:
- other: culture medium
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 °C
- pH:
- 8.0
- Nominal and measured concentrations:
- 3.125, 6.25, 12.5, 25 and 50 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass conical flask (250 mL) containing 100 mL test solution and loosely stoppered to reduce evaporation. All flask were incubated and shaken (approximately 100 rpm) in an orbital shaker.
- Initial cells density: 15500 cells/mL
- Control end cells density: 262000 cells/mL
- No. of vessels per concentration (replicates): 3
OTHER TEST CONDITIONS
- Adjustment of pH: 8
- Photoperiod: continuous ( approximately 7000 lux)
TEST CONCENTRATIONS
- Test concentrations: 3.125, 6.25, 12.5, 25 and 50 mg/L - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The data mean cell density of control at 0 h is 15500 cells/mL, mean cell density of control at 72h is 262000 cell/mL. The data show that cell concentration of control cultures increased by factor of 17 during the test in line with the OECD Guideline that status the environment must be at least by a factor of 16 after 72 hours.
All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control test cultures. - Validity criteria fulfilled:
- yes
- Conclusions:
- For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.
- Executive summary:
The study was performed to assess the growth inhibition of test item to Scenedesmus subspicatus according to the OECD Guideline for Testing of Chemicals No. 201 (2006).
All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control test cultures. For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.
Reference
Description of key information
One read-across study is available.
For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 14 mg/L
Additional information
One read-across study is available which was performed to assess the growth inhibition of test item to Scenedesmus subspicatus according to the OECD Guideline 201.
All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control test cultures. For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.