5-[[p-(benzylmethylamino)phenyl]azo]-1,4-dimethyl-1H-1,2,4-triazolium chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Further mutagenicity studies on pesticides in bacterial reversion assay systems

Author:

M. Moriya, T. Ohta, K. Watanabe, T. Miyazawa, K. Kato and Y. Shirasu

Year:

1983

Bibliographic source:

Mutation Research, 116 (1983) 185-216

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

Ames assay was performed to determine the mutagenic nature of Benzyladenine

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

6- Benzyladenine

Cas Number:

1214-39-7

Molecular formula:

C12H11N5

IUPAC Name:

6- Benzyladenine

Details on test material:

- Name of test material: Benzyladenine
- IUPAC name: 6- Benzyladenine
- Molecular formula: C12H11N5
- Molecular weight: 225.254 g/mol
- Substance type: Organic

Specific details on test material used for the study:

- Name of test material: Benzyladenine
- IUPAC name: 6- Benzyladenine
- Molecular formula: C12H11N5
- Molecular weight: 225.254 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Method

Target gene:

Histidine

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium, other: TA 100, TA98, TA 1535, TA 1537 and TA 1538

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

E. coli WP2

Remarks:

hcr

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

No data

Metabolic activation:

with and without

Metabolic activation system:

S9 metabolic activation system

Test concentrations with justification for top dose:

up to a dose of 5000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: Water or DMSO
- Justification for choice of solvent/vehicle: Chemical solubility

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Preincubation period: No data
- Exposure duration: 2 days
- Expression time (cells in growth medium): 2 days
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: No data

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data

OTHER: No data

Rationale for test conditions:

No dataplate

Evaluation criteria:

The plates were observed for a dose dependent increase in the number of revertants/plate

Statistics:

No data

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium, other: TA 100, TA98, TA 1535, TA 1537 and TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2

Remarks:

hcr

Metabolic activation:

with and without

Genotoxicity:

not specified

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

No data

Remarks on result:

other: No mutagenic potential

Applicant's summary and conclusion

Conclusions:

Benzyladenine did not induce gene mutation in Salmonella typhimurium TA 100, TA98, TA 1535, TA 1537 and TA 1538 and Escherichia coli WP2 hcr in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant in vitro.

Executive summary:

Ames assay was performed to determine the mutagenic nature of Benzyladenine. The study was performed as per the method described by Ames et al. The test chemical was dissolved in either DMSO or water depending on solubility and used up to a dose level of 5000µg/plate using Salmonella typhimrium strains TA 100, TA98, TA1535, TA1537 and TA1538 and E. coli WP2 hcr. Concurrent solvent and positive control chemicals were included in the study.The plates were observed for a dose dependent increase in the number of revertants/plate. Benzyladenine did not induce gene mutation inSalmonella typhimurium TA 100, TA98, TA 1535, TA 1537 and TA 1538 and Escherichia coli WP2 hcr in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant in vitro.