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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study. However, no information on the preparation of the test media was given, but scientifically acceptable.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: 79/831 EWG, Attachment V, C2
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylbut-3-en-2-ol
EC Number:
204-068-4
EC Name:
2-methylbut-3-en-2-ol
Cas Number:
115-18-4
Molecular formula:
C5H10O
IUPAC Name:
2-methylbut-3-en-2-ol
Details on test material:
- Name of test material (as cited in study report): 2-methylbuten-3-ol-2
- Lot/batch No.: 88/70
- Analytical purity: > 99 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of the test item of the highest test concentration (nominal 500 mg/L) was prepared. Then, adequate volumes of this stock solution were diluted with culture medium to obtain the desired test concentrations.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.70 +/- 0.50 mmol/l
Test temperature:
19 - 21 °C
pH:
8.0 to 8.1
Dissolved oxygen:
8.5 to 8.9 mg/l
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
control,
62.2,
125,
250,
500
Details on test conditions:
TEST SYSTEM
- Test vessel: Test tubes with flat bottom (glass)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water :A synthetic fresh water was used for culture and test purposes. Properties of this medium: Ratio Na:K: 10 : 1; pH value: 7.7 - 8.3. After preparation the medium was aerated for approximately 24 h until saturation.
- Alkalinity: up to pH 4.3: 0,80 +/- 0.10 mmol/l
- Ca/mg ratio: 4.1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16 light : 8 h dark
- Light intensity: Artificial light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Swimming ability of the test animals after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

The number of mobile daphnids after hours of exposure at the different  test concentrations was as follows:

Concentration Mobile daphnia after x hours
[mg/l] 0 3 6 24 48
Control 20 20 20 20 20
62.5 20 20 20 20 20
125 20 20 20 20 20
250 20 20 20 19 19
500 20 20 20 20 20

The calculated EC50 (48 h) was > 500 mg/l (confidence limits 95%: not determined). The EC0 (48 h) was 500 mg/L and the EC100 (48 h) was > 500 mg/L.

The calculated EC50 (24 h) was > 500 mg/l (confidence limits 95%: not determined). The EC0 (24 h) was 500 mg/L and the EC100 (24 h) was > 500 mg/L.

All results refer to nominal test concentrations.

Applicant's summary and conclusion

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