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EC number: 209-506-8 | CAS number: 583-52-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 - 29 Jul 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2006
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Department of Health of the government of the United Kingdom
- Specific details on test material used for the study:
- Storage conditions: ambient temperature (10 to 30 °C) "
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations and sampling method: samples of test media of all concentrations and the control were analysed at 0 (fresh media), 24, 48 and 72 h. At 72 h, inoculated replicate test vessels at each treatment level were pooled prior to sampling.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a 100 mg/L solution was prepared by diluting the substance directly in the medium. The remaining test concentrations were prepared through serial dilution of this solution. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: 278/4
- Source: laboratory culture
ACCLIMATION
- Acclimation period: prior to testing, duplicate cultures were prepared and incubated under test conditions for at least 72 h
- Culturing media and conditions: same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- -
- Test temperature:
- 21.0 – 21.9 °C
- pH:
- 7.45 - 7.95
- Dissolved oxygen:
- -
- Salinity:
- -
- Conductivity:
- -
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 10, 18, 32, 56 and 100 mg/L
Mean measured:- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL conical flasks
- Fill volume: 100 mg/L
- Initial cells density: 1 x 10^4 cells/mL
- Control end cells density: 54.8 cells x 10^4/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Ca/Mg ratio: 18 mg/L CaCl2 2H2O per 15 mg/L MgSO4 7H2O
- Intervals of water quality measurement: temperature was recorded continuously
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuous light conditions
- Light intensity and quality: 4440-8880 Lux (LED lights)
- Agitation: at ca 100 rpm
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: every 24 h with a particle counter (Z2 Coulter Counter®)
TEST CONCENTRATIONS
- Range finding study; yes
- Test concentrations: 0 (control), 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: the results of the range-finding test suggested that the 72-h EC50 value would be greater than 100 mg/L and the NOEC may be between 10 and 100 mg/L- Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes (54 fold)
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: freshly prepared media appeared as colourless solutions however, the 100 mg/L test concentration was slightly cloudy a period of time. After 24 h, slight precipitates were observed at concentrations
of 32, 56 and 100 mg/L. However, after gentle shaking the precipitate re-dissolved.Table 1: Analytical results.
Nominal
concentration
(mg/L)0 h % nominal 24 h % nominal 48 h % nominal 72 h % nominal Time weighted
mean
measured
concentration
(mg/L)Control <LOQ - <LOQ - <LOQ - <LOQ - - 10 8.88 89 8.35 84 11.42 114 6.45 65 8.8 18 17.46 97 19.74 110 13.92 77 12.86 71 16 32 20.69 65 25.25 79 24.25 76 33.24 104 26 56 47.71 85 42.23 75 56.63 101 52.21 93 50 100 76.1 76 82.4 82 75.99 76 77.68 78 78 Since some of the measured concentrations were < 80% of the nominal value, it was considered appropriate to base the results on mean measured test concentrations.
Table 2: Cell concentrations of Pseudokirchneriella subcapitata during exposure time.
Measured
concentration
(mg/L)Mean cell concentration
(cells x 10^4/mL)24 h 48 h 72 h Control 3.02 13.4 54.8 8.8 2.44 12.7 54.8 16 2.77 13 59.4 26 2.7 12.4 57 50 2.32 12.1 51.6 78 2.79 14.5 69.9 Table 3: % Inhibition of growth rate of Pseudokirchneriella subcapitata relative to the control.
Measured
concentration
(mg/L)Reduction in average area under the
growth curve relative to the control (%)Reduction in average specific growth
rates relative to the control (%)0-24 h 0-48 h 0-72 h 0-24 h 0-48 h 0-72 h Control - - - - - - 8.8 29 11 3 19 2 0 16 12 5 -4 8 1 -2 26 16 10 1 10 3 -1 50 35 17 9 24 5 2 78 11 -4 -20 7 -3 -6 The mean coefficient of variation for section-by-section specific growth rates (0-24, 24-48 and 48-72 h) in the control culture was 8.51%.
The coefficient of variation for average specific growth rates during the whole test period in replicate control culture was 3.17%.
Table 4: Further toxicity results.
Toxicity values (mg/L) 72-hour Parameter Yield >78 EyC10 >78 EyC20 >78 EyC50 >78 NOEC ≥78 Biomass EbC10 >78 EbC20 >78 EbC50 >78 NOEC ≥78 Growth Rate ErC20 >78 NOEC ≥78 - Validity criteria fulfilled:
- yes
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the analogue justification provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Referenceopen allclose all
Description of key information
ErC50 (72 h) > 78 mg/L and NOErC (72 h) ≥78 mg/L (geom. mean meas., OECD 201) based on read across from CAS 6009-70-7
Key value for chemical safety assessment
Additional information
No data are available on the toxicity of dipotassium oxalate anhydrate (CAS 583-52-8) to algae. Therefore, a read across was performed to the analogues substance diammonium oxalate monohydrate (CAS 6009-70-7). Details on the read across justification are provided in section 13 of the IUCLID dossier.
The study was conducted according to the OECD guidance 201 and GLP standards (reference: 6.1.5-1). In a static system the test organism Pseudokirchneriella subcapitata was exposed for 72 h to nominal test concentrations of 0 (control), 10, 18, 32, 56 and 100 mg/L (corresponding to <LOQ, 8.8, 16, 26, 50 and 78 mg/L geom. mean meas.). An analytical monitoring was performed via LC-TOF/MS. Analysis of the test media samples was conducted after 0, 24, 48 and 72 h. The recovery of the test substance was between 65% and 114% of the nominal values. Therefore the results refer to geom. mean measured concentration. No toxic effects were observed up to the highest tested concentration resulting in an ErC50 (72 h) of > 78 mg/L and a NOErC (72 h) ≥ 78 mg/L (geom. mean meas.).
Based on the results of the read across substance, no toxic effects to algae are expected by dipotassium oxalate.
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