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Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report date:
1976

Materials and methods

Principles of method if other than guideline:
BASF internal testing method
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, amino-, reaction products with 1-amino-9,10-anthracenedione and tetrabromo-8,16-pyranthrenedione
EC Number:
215-520-5
EC Name:
Anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, amino-, reaction products with 1-amino-9,10-anthracenedione and tetrabromo-8,16-pyranthrenedione
Cas Number:
1328-25-2
Molecular formula:
C126H58N4O10
IUPAC Name:
anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, amino-, reaction products with 1-amino-9,10-anthracenedione and tetrabromo-8,16-pyranthrenedione
Test material form:
liquid
Details on test material:
Vat Black 9

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
no data

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: NaCl
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 mg
Duration of treatment / exposure:
1 hour
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
no data
Details on study design:
Animals were observed after 1, 24 hours and 8 days.

SCORING SYSTEM:
Erythema and Edema
Ø - no effect
(+) - questionable
+ - mild
++ - moderate
+++ - marked

R - erythema
Ö - edema
Sch - desquamation
N - necrosis
Tr - opacity
Na - scar

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 8 d
Score:
0
Max. score:
4
Remarks on result:
no indication of irritation
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 8 d
Score:
0
Max. score:
2
Remarks on result:
no indication of irritation
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 8 d
Score:
0
Max. score:
3
Remarks on result:
no indication of irritation
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 8 d
Score:
0
Max. score:
4
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
Within the first hour, slight redness occurred. All symptoms disappeared within 24 d. No signs of corrosion observed.

The positive control (NaCl) caused no irritation.

Any other information on results incl. tables

Table 1: Results on Eye Irritation

Findings after
1 hour 24 hours 8 days
Test item R+/black substance residues  Ø Ø
NaCl  Ø  Ø Ø

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
The substance caused no significant irritation in rabbit eyes. The substance is not classifiable according to CLP criteria.