Registration Dossier
Registration Dossier
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EC number: 208-144-8 | CAS number: 512-56-1
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Trimethyl phosphate
- EC Number:
- 208-144-8
- EC Name:
- Trimethyl phosphate
- Cas Number:
- 512-56-1
- Molecular formula:
- C3H9O4P
- IUPAC Name:
- trimethyl phosphate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Purity test date: > 99 %
Method
- Target gene:
- His
Trp
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S-9 fraction from Phenobarbital and 5,6-Benzoflavone pretreated male SD rats with NADPH-generating system
- Test concentrations with justification for top dose:
- 0, 10, 50, 100, 500, 1000, 5000 μg/plate
- Details on test system and experimental conditions:
- Procedure: Plate incorporation method
Plates/test: 3
No. replicates: 2
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: Cytotoxicity conc: With and without metabolic activation: 5000 μg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: Cytotoxicity conc: With and without metabolic activation: 5000 μg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The Bacterial reverse mutation assay with Trimethyl phosphate with and without metabolic activation is negative.
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