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EC number: 201-999-8
CAS number: 90-50-6
The objective of the study was to evaluate 3,4,5-TRIMETHOXYCINNAMIC ACID
for its ability to generate acute toxic effects on the mobility of Daphnia
magna during an exposure period of 48 hours and, if possible, to
determine the EC50at 24 and 48 hours of exposure.
The study procedures described in this report were based on the OECD
guideline No. 202, 2004.
The batch of 3,4,5-TRIMETHOXYCINNAMIC ACID tested was a light yellow
crystalline solid with a purity of approximately 99% and completely
soluble in test medium at the concentrations tested.
Preparation of test solutions started with the highest concentration of
100 mg/L applying a 10-minute period of ultrasonic waves followed by a
20-minute period of magnetic stirring to accelerate dissolution of the
test item in medium. Lower test concentrations were prepared by
subsequent dilutions of the highest concentration in test medium. All
test solutions were clear and colorless at the end of the preparation
A combined limit/range-finding test was performed. wenty
daphnids per group (5 per replicate, quadruplicate) were exposed to an
untreated control and to 100 mg/L, in a limit test. In addition, ten
daphnids per group (5 per replicate, duplicate) were exposed to 0.10,
1.0 and 10 mg/L in the combined range-finding test. The total exposure
period was 48 hours and samples for analytical confirmation of exposure
concentrations were taken at the start and at the end of the test.
No daphnids became immobilized in the control and in the three lowest
test concentrations. After 24 hours 25% of the daphnids were immobilized
at the highest test concentration (100 mg/l). This increased to 35%
immobilization after 48 hours.
The study met the acceptability criteria prescribed by the study plan
and was considered valid. Samples taken from the highest test
concentration were analysed. The measured concentrations were at the
level of nominal throughout the test duration (94-96%).
48h-EC50for Daphnia magna exposed to
3,4,5-TRIMETHOXYCINNAMIC ACID exceeded an analytically confirmed nominal
concentration of 100 mg/L.
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