2-amino-2-methylpropane-1,3-diol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: screening test, other

Remarks:

Modified MITI Test (II), according to OECD Guideline 302C.

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 Jan - 24 Feb 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: municipal sewage treatment plant Reutlingen
- Pretreatment: twice washed with dechlorinated tap water and centrifuged; dry weight of centrifuged sludge is 60 mg/g wet sludge
- Concentration of sludge: in the test a concentration of 100 mg/L dry weight is used

Duration of test (contact time):

22 d

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: composition according to OECD 302C (6 instead of 3 mL per solution are used)
- Test temperature: 25 ± 1 °C
- pH: 7.0
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: 100 mg/L

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 4
- Measuring equipment: BOD measuring device BSB 1002, company WTW
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: CO2 absorption with KOH

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 replicates
- Abiotic sterile control: yes, 3 replicates
- Toxicity control: yes, 3 replicates

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

96.7

Sampling time:

22 d

Results with reference substance:

Degradation after 22 d: 74.9 %

The BOD (22 d) of the test substance is 61.9 mg O2/L (less blank and abiotic control). The ThOD of the test substance is 64 mg O2/L.

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

Degradation of the test substance after 22 d: 96.7 %. The substance is inherently biodegradable in the Modified MITI Test (II), according to OECD Guideline 302C.

Executive summary:

The substance is inherently biodegradable in the Modified MITI Test (II), according to OECD Guideline 302C.

Description of key information

The substance is inherently biodegradable in the Modified MITI Test (II), according to OECD Guideline 302C.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable