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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Study period:
12 Sept. 1973 to 03 Sept. 1974
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1974
Report date:
1974

Materials and methods

Principles of method if other than guideline:
According to BASF-internal standard: 5 NMRI mice (per sex per dose) were exposed via intraperitoneal route at a range of concentrations 800-3200 mg.kg b.w.. Observation period was 7 resp. 14 d.
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Amines, C10-14-branched and linear alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
EC Number:
285-083-3
EC Name:
Amines, C10-14-branched and linear alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
Cas Number:
85029-58-9
Molecular formula:
C34H24CrN8O6.C10-14H21-29NH2
IUPAC Name:
Amines, C10-14-branched and linear alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
Details on test material:
The substance was formerly identified as: 73297-13-9 / 615-953-3 / Chromate(1-), bis[2-[2-[4,5-dihydro-3-methyl-5-(oxo-κO)-1-phenyl-1H-pyrazol-4-yl]diazenyl-κN1]benzoato(2-)-κO]-, hydrogen, compd. with 1-tridecanamine (1:1:1)
Specific details on test material used for the study:
Name of the test substance used in the study report: Neozapongelb R (TDA) Chromium-1.2-complex

performed under old CAS 73297-13-9
new CAS 85029-58-9, same structure

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female
Details on test animals or test system and environmental conditions:
Mean body weight: males 22.2 g, females 21.7 g

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
CMC (carboxymethyl cellulose)
Details on exposure:
Aqueous suspension with CMC
Test concentrations used: 8 and 16% (G/V)
Due to technical reasons, a higher test substance concentration (more than 16%) could not be applied.
Doses:
800, 1600, 2000, 2500, 2800, 3200 mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 resp. 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 2 600 mg/kg bw
Remarks on result:
other: 7-day LD50 value
Mortality:
800 mg/kg: no deaths after 7 days; 1600 mg/kg: 1/10 after 7 days; 2000 mg/kg: no deaths after 7 days; 2500 mg/kg: 3/10 after 7 days; 2800 mg/kg: 8/10 after 14 days; 3200 mg/kg: 10/10 after 7 days
Clinical signs:
Accelerated, partly intermittent breathing, partly crouched position, atoniy, spastic gait, stretching. On the 3rd, 6th, 7th and 12th study, day no signs were observed on the surviving animals. Late deaths.
Gross pathology:
Intra-abdominal substance residues and adherences

Any other information on results incl. tables

Dose mg/kg

Konc. %

Tierzahl

1 h

24 h

48 h

7 d

14 d

3200

16

5 M

5 W

0/5

0/5

2/5

0/5

3/5

2/5

5/5

5/5

5/5

5/5 

2800

16

5 M

5 W

0/5

0/5

0/5

0/5

2/5

0/5

4/5

4/5

4/5

4/5

2500

16

5 M

5 W

0/5

0/5

0/5

0/5

1/5

0/5

2/5

1/5

2/5

1/5

2000

16

5 M

5 W

0/5

0/5

0/5

0/5

0/5

0/5

0/5

0/5

0/5

0/5

1600

16

5 M

5 W

0/5

0/5

0/5

0/5

0/5

0/5

1/5

0/5

1/5

0/5

800

8

5 M

5 W

0/5

0/5

0/5

0/5

0/5

0/5

0/5

0/5

0/5

0/5

Applicant's summary and conclusion

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