Pentan-3-one

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1954

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable range finding test.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-Finding Toxicit Data List V

Author:

Smyth HF, Carpenter CP, Weil CS & Pozzani UC

Year:

1954

Bibliographic source:

Arch. Ind. Hyg Occup. Med. 10: 61–68

Reference 2

Reference Type:

publication

Title:

Range-Finding Toxicity Data List VI

Author:

Smyth HF, Carpenter CP, Weil CS, Pozzani UC, Striegel JA

Year:

1962

Bibliographic source:

Am. Ind. Hyg. Assoc. J. , 23. 95-107, 1962

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

Method: according to Draize, J.H. et al.: J. Pharmacol. & Exper. Therap. 82, 377

GLP compliance:

no

Remarks:

prior to GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pentan-3-one

EC Number:

202-490-3

EC Name:

Pentan-3-one

Cas Number:

96-22-0

Molecular formula:

C5H10O

IUPAC Name:

pentan-3-one

Details on test material:

- Name of test material: Pentanone-3

Test animals

Species:

rabbit

Strain:

other: New Zealand giant albino

Sex:

male

Details on test animals or test system and environmental conditions:

- weight of animals: 2.5-3.0 kg

Administration / exposure

Type of coverage:

occlusive

Vehicle:

not specified

Details on dermal exposure:

no data

Duration of exposure:

24 h

Doses:

no data

No. of animals per sex per dose:

4

Control animals:

no

Details on study design:

Penetration of rabbit skin is estimated by a technique essentially the one-day cuff method of Draize and associates using groups of four male New Zealand giant albino rabbits weighing 2.5 to 3.5 kg. The fur is closely clipped over the entire trunk, and the dose, retained beneath an impervious plastic film, contacts about 1/10 of the body surface . Dosages greater than 20 mL per kilogram of body, weight cannot be retained in contact with the skin . After 24 hours' contact the film is removed, and mortality is considered complete after 14 additional days.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

16 200 mg/kg bw

Remarks on result:

other: LD50 obtained graphically

Mortality:

only LD 50 value of 20 ml/kg bw (corr. to 16200 mg/kg bw) reported

Clinical signs:

other: no data

Gross pathology:

no data

Other findings:

no data

Applicant's summary and conclusion

Executive summary:

In this acute dermal toxicity study (Smyth et al., 1954) groups of four male giant albino rabbits were dermally exposed to Diethyl ketone occlusively for 24 hours to an area of 1/10 of the body surface. Animals then were observed for 14 days. A LD50 value of 20 mL/kg bw was identified graphically which corresponds to 16.2 g/kg bw. This study is classified as acceptable key study.