Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1998

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Analytical Purity: > 99 %

Test solutions

Vehicle:
yes
Details on test solutions:
TWEEN 80 was used as dispersant for the test item

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Strain: Hamilton-Buchanan
- Source: West Aquarium Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (mean, range and SD): 2.8 cm, 2.5 - 3.4 cm, 0.19 cm
- Method of breeding: 22 +- 1 °C; O2-content >= 80% ; 12 h day/night cycle, 700 Lux; <= 1g fish/l water;
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period:14 days
- Acclimation conditions: same as test
- Type and amount of food:
- Food type: Tetra Min, Tetra Werke, Melle, Germany;
- Amount: ad libitum;
- Frequency: 2 times a day;

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
Test groups: 21.0 - 23.0 °C
Control groups: 21.2 - 23.0 °C

pH:
Test groups: 7.0 - 8.0
Control groups: 7.2 - 8.1

Dissolved oxygen:
Test groups: 4.6 - 9.4 mg/l
Control groups: 6.9 - 9.6 mg/l


Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
13.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 11.8 - 16.8
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
13.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 11.5 - 16.2
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.

Applicant's summary and conclusion

Conclusions:
From this study, LC0 (48 h and 96 h) for 2-chlorobenzal chloride was determined to be 10 mg/L, LC50 (48 h and 96 h) to be 13.9 mg/L and 13.5 mg/L and LC100 (48h and 96 h) to be 35 mg/L.