3,3'-[[4-[(2,6-dichloro-4-nitrophenyl)azo]phenyl]imino]bispropiononitrile

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From September 16 to 30, 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

14 d instead of 28 d period

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Version / remarks:

1981

Deviations:

not applicable

Remarks:

test duration in 1981 version was 14 days only

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-[[4-[(2-chloro-4-nitrophenyl)azo]phenyl]imino]bis[propiononitrile]

EC Number:

223-765-4

EC Name:

3,3'-[[4-[(2-chloro-4-nitrophenyl)azo]phenyl]imino]bis[propiononitrile]

Cas Number:

4058-30-4

Molecular formula:

C18H15ClN6O2

IUPAC Name:

3,3'-({4-[(2-chloro-4-nitrophenyl)diazenyl]phenyl}imino)dipropanenitrile

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): mixture of polyvalent bacteria collected 14 September 1993 in the aeration tank of a domestic sewage treatment plant, ARA
Basel Communal.
- Concentration of sludge: 30 mg TS/L

Duration of test (contact time):

ca. 14 d

Initial test substance concentration

Initial conc.:

ca. 100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 20±2°C
- Continuous darkness: yes

Reference substance

Reference substance:

other: Natriumbenzoat

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 0

Sampling time:

14 d

Remarks on result:

other: no biodegradation observed

BOD5 / COD results

Results with reference substance:

The % of BOD versus ThOD is : 86 % (14 days)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The substance was not found to be readily biodegradable (0% after 14 d based on O2 consumption). In the toxicity control, a biodegradation level of 84% after 14 d was found clearly showing that the test item is not inhibitory to activated sludge activation.

Executive summary:

Method

The inherent biodegradability of test substance was determined in a 14 days modified MITI-test according to the OECD Guideline for Testing of Chemicals, No. 301C, 1981.

 

Result

The biodegradation of the test substance (corrected by the Incoculum control) expressed as the % of BOD versus ThOD is : 0 % (14 days)