Biphenyl-2-ol, ethoxylated, esters with acrylic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Adopted : 17 July, 1992

Deviations:

no

Remarks:

The study was performed in accordance with the study plan and guideline, and no deviation took place during the test period.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Ethoxylated-o-phenylphenol acrylate; Lot No 71016M95. Appearance: Clear liquid; Purity : 97.4 %; Supplier : Miwon Commercial Co., Ltd

Analytical monitoring:

no

Details on sampling:

The mortality and behavior of test species were evaluated at times, 1,4,24,48,72 and 96 hours.

Details on test solutions:

The concentration range was selected on the basis of a range finding study, were a LC50 of 10 mg/l were indicated.
the concentration range was 1.0-21.5 mg/l, spaced by a factor of 2.15

Test organisms (species):

Oryzias latipes

Details on test organisms:

The ricefish (Oryzias latipes ) are recommended species by OECD guidelines for the testing of chemicals 203 “Fish acute toxicity test” (Adopted : 17 July 1992) and was selected for this test because of a plenty of comparing toxicological data.
132 fish were transferred into 50 L bath 7 days before the test started. Then the acclimation was began under same condition as test environment. The acclimated fish were used in the test, because no dead fish was observed and all fish were healthy during the acclimation. The food was not given for 24 h immediately prior to exposure.
The ricefishes were bred in 100 L rectangular water bath (59 cm W. × 40 cm D. ×39 cm H.). The temperature of breeding water was maintained between 21 ~ 25 oC and photo periods were 16 h light and 8 h dark. Feeds were supplied two times a day; Tetramin flake (Tetra Co. Germany) in the morning, Blood worm (Hikari sales USA Inc., USA) in the afternoon.
The Temperature of ricefish breeding room and breeding water were recorded automatically every 30 minutes. Photo periods were recorded every months. As the results of the monitoring, there was not observed an alteration effecting the test.
Ground water used as breeding water was filtered through 1.0 μm pre-filter and 0.2 μm micro-filter and then used after aeration over 24 h in the test water tank. The water quality was analyzed according to drinking water test guideline every 6 months. As the result of the analysis, there was not observed the alteration effecting the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Total hardness of the test water 96.34 mg CaCO3/L (measured by KS M 0100); Alkanity: 70.47 mg/L (measured by HCl titration method in the standard method (21th edition, APHA, AWWA, WEF, USA))

Test temperature:

Mean: 21.6 (21.0-22.6) oC

pH:

Mean: 8.03 (7.71-8.39) (PP-15 pH meter, Sartorius)

Dissolved oxygen:

7.05 (5.69-8.38) mg/l (Dissolved oxygen (810A+ DO meter, Orion Research Incorporation))

Salinity:

Freshwater

Nominal and measured concentrations:

Concentrations of the Ethoxylated-o-phenylphenol acrylate in test solutions were 0.91, 1.34, 2.09, 3.45, 5.09 and 9.02 mg/L (nominal concentrations 0.95, 1.53, 2.44, 3.91, 6.25 and 10.00
mg/L)

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1.809 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.514 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

No reference substance was applied

Reported statistics and error estimates:

Determinations of LC50 was based on measured concentration.
The 96-LC50 value was determined as 1.514 mg/L with (1.285 – 1.809) mg/L of 95 % confidence limits.
The 48 h-LC50 value was determined as 1.809 with (1.506 – 2.190) mg/L of 95 % confidence limits.
The NOEC was 0.86 mg/L at 48 and 96 h.

Validity criteria fulfilled:

yes

Conclusions:

The 96-LC50 value to Oryzias latipes of Ethoxylated-o-phenylphenol acrylate was determined as 1.514 mg/L with (1.285 – 1.809) mg/L of 95 % confidence limits.
The 48 h-LC50 value to Oryzias latipes of Ethoxylated-o-phenylphenol acrylate was determined as 1.809 with (1.506 – 2.190) mg/L of 95 % confidence limits.
The NOEC to Oryzias latipes of Ethoxylated-o-phenylphenol acrylate was 0.86 mg/L at 48 and 96 h.

Executive summary:

The short-term toxicity of Ethoxylated-o-phenylphenol acrylate to the Oryzias latipes, was tested according to OECD Guidelines 203 following GLP.

The LC50 values were established to 1.81 mg/l (48h) and 1.51 mg/l (96h) (Determinations of LC50 was based on measured concentration.).

Description of key information

One study was identified concerning toxicity to a fresh water fish, no marine data is available

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.51 mg/L

Additional information

The short-term toxicity of Ethoxylated-o-phenylphenol acrylate to the Oryzias latipes, was tested according to OECD Guidelines 203.

The LC50 values were established to 1.81 mg/l (48h) and 1.51 mg/l (96h).