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EC number: 643-080-8 | CAS number: 24389-25-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- other: Testing method relating to the new chemical substances, etc. (Yakushokuhatsu No. 1121002, Heisei 15-11-13 Seikyoku No. 2, Kanpokihatsu No. 031121002 on November 21, 2003).
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- To assess the toxicity of the test substance by conducting acute swimming immobilization test in Daphnia magna for notification of the new chemical substance under the Chemical Substance Control Law (CSCL) in Japan.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- During the study period, the test substance was stored in the desiccators for storage of the test substance in Yokohama Research Center (storage conditions: room temperature, under nitrogen purging).
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 4, 6, 8.9, 13, 20 mg/L
- Sampling method: The middle layer of the test solution was collected from the vessels for measurement of the test environment of each test concentration group at the start of exposure and after renewal of test solution, from one vessel of each test concentration group before renewal of the test solution and at the end of exposure.
- Sample storage conditions before analysis: Samples not stored analysis straightaway - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Eluate: Elendt M4 medium
- Controls: test water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): none
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia
- Source: The Environment Agency, National Institute for Environmental Studies, Japan (subcultured at Yokohama Research Center)
- Age of parental stock (mean and range, SD): Female juveniles (<24h old)
- Feeding during test: None
Rearing conditions of parent Daphnia to obtain the test organisms:
The young daphnids derived from parent animals held under the following conditions were used for the test.
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris (single-cell green algae) 6 mg C (organic carbon content)/2 L/day
- Feeding frequency: daily
- Health during acclimation (any mortality observed): none
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 236 CaCO3/L
- Test temperature:
- 20 +/- 1°C
- pH:
- 6.0 - 9.0
- Dissolved oxygen:
- 3 mg/L and above, without aeration
- Salinity:
- Not applicable
- Conductivity:
- Not applicable
- Nominal and measured concentrations:
- Nominal: 4, 6, 8.9, 13 and 20 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beaker
- Type (delete if not applicable): open / closed: closed covered with teflon sheet and lid to prevent evaporation
- Material, size, headspace, fill volume: Glass, 100 mL, no headspace filled to top of vessel
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): test solutions renewed after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
Elendt M4 medium (formulated water prescribed in OECD Guideline for Testing of Chemicals 202 (2004) Daphnia sp., Acute Immobilisation Test ) was sufficiently aerated and used for the test at the adjusted temperature of 20 ± 1°C. Theoretical hardness was 250 mg CaCO3/L. The hardness and pH of the test solution were measured before the start of exposure and they were 236 mg CaCO3/L and 8.4, respectively, and it was confirmed that these values were within the appropriate range (hardness: 250 mg CaCO3/L or below, pH: 6.0 to 9.0).
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: Room light, 16 hours light , 8 hours dark
- Light intensity: 800 lux or less
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Frequency: At 24 hours (before the renewal of the test solution) and 48 hours after the start of exposure
Parameter: Number of immobilized test organisms*1, presence/absence of other abnormality*2
*1: Daphnia that could not swim for 15 seconds after gentle agitation of the test vessel were considered to be immobilized.
*2: Abnormal symptom in the activity or appearance (discoloration, etc.,) and daphnia floating on the water surface were recorded.
RANGE-FINDING STUDY
- Test concentrations: 0 (control), 2, 4, 20 and 32 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 3.44 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- none
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.48 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL
- Remarks:
- 5.87-7.09 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 11.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 0.76 mg/L
- performed every 6 month to test sensitivity of Daphnia - Reported statistics and error estimates:
- Probit analysis
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is toxic to aquatic invertebrates with a 48h EC50 value of 6.48 mg/L.
Reference
Table 1 Measured concentrations of the test substance in test water
Test group | Nominal Concentration | Measured Concentrations (mg/L) | ||||
0 Hour New |
24 Hours Old |
24 Hours New |
48 Hours Old |
Mean (TWA) | ||
(Percent of nominal, %) | ||||||
Control | - | <0.005 | <0.005 | <0.005 | <0.005 | - |
1 | 4.0 | 3.51 (88) |
3.53 (88) |
3.43 (86) |
3.31 (83) |
3.44 (86) |
2 | 6.0 | 5.01 (84) |
4.92 (82) |
5.04 (84) |
4.98 (83) |
4.99 (83) |
3 | 8.9 | 7.55 (85) |
7.38 (83) |
7.94 (89) |
7.68 (86) |
7.64 (86) |
4 | 13.0 | 11.3 (87) |
10.7 (82) |
11.3 (87) |
11.2 (86) |
11.1 (85) |
5 | 20 | 18.2 (91) |
17.1 (86) |
17.7 (89) |
17.5 (88) |
17.6 (88) |
Table 2: no. of immobilized Daphnia magna (% immobility)
Test group | Nominal Concentration (mg/L) |
Mean Measured Concentration (mg/L) |
Cumulative no. of immobilized Daphnia (% Immobility) |
|
24 hours | 48 hours | |||
Control | - | - | 0 (0) | 0(0) |
1 | 4.0 | 3.44 | 0 (0) | 0 (0) |
2 | 6.0 | 4.99 | 0 (0) | 1 (5) |
3 | 8.9 | 7.64 | 0 (0) | 17 (85) |
4 | 13 | 11.1 | 0 (0) | 20 (100) |
5 | 20 | 17.6 | 20 (100) | 20 (100) |
Description of key information
In an GLP-compliant study conducted with analytical monitoring in accordance with a standard method equivalent to the OECD guideline, the toxicity of the test item to aquatic invertebrates (Daphnia magna) was assessed. The 48h EC50 value was determined to be 6.48 mg/L indicating that the substance is toxic to aquatic invertebrates. The 48h EC50 value is used for the Chemical Safety Assessment.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 6.48 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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