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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23.12. - 27.12.1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented study without GLP

Data source

Reference
Reference Type:
publication
Title:
Acute toxicities of organic chemicals to Fathead minnows (Pimephales promelas)
Author:
Brooke, L.T., Call, D.J., Geiger, D.L. & Northcott, C.E.
Year:
1984
Bibliographic source:
ISBN 0-9614968-0-0

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not applicable
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Chemical Source: Aldrich Chemical Co, Milwaukee, USA
Purity: 97 %
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data available

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
All test exposure chambers were sampled at approximately mid-depth at 0 and 96 hr and one of each duplicate exposure chamber at 24, 48 and 72 hr

Test solutions

Vehicle:
not specified
Details on test solutions:
no data available

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
Fathead minnows used in the tests were cultured from brood stock provided by the U.S. ERA Environmental Research Laboratory-Duluth. Adults were held at 25 C in flowing water with a 16 hr light-controlled photoperiod and fed frozen adult brine shrimp (Artemia sp.).
Age at test start: 28-31 days, mean length: 21.1 mm, mean weight: 0.172 g

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no data available

Test conditions

Hardness:
45.4 mg/L CaCO3
Test temperature:
24.0 °C
pH:
7.60
Dissolved oxygen:
6.9 mg/L
Salinity:
39.5 as CaCO3
Nominal and measured concentrations:
nominal: 0, 2.6, 4.3, 7.2, 12.0, 20.0
analysed: 0, 0.73, 1.4, 2.7, 5.5, 12.3
Details on test conditions:
Exposures were conducted in two-L battery jars. Test solutions were renewed by siphoning 90% of the solutions from the jars and replacing them with fresh stock of the appropriate concentration. The exchange of solutions was done at least every 48 hr.
Fish were not fed during chemical exposures. During exposure to toxicants the fish were routinely observed for behavioral responses (effects) and deaths.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 2.0-2.4 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
2.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks on result:
other: 95% CL: 2.0-2.4 mg/L
Details on results:
affected fish stopped schooling, swam at the water surface and lost equilibrium prior to death.
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Observations of affected and dead fish exposed to test item were combined when duplicate exposures were made, resulting in a single LC50 a estimate per test. Estimation of the LC50 was made using the "trimmed Spearman-Karber" method (Hamilton et_ al_., 1977).

Applicant's summary and conclusion

Conclusions:
Based on the test results, the 96-hour LC50 for fathead minnow (Pimephales promelas) for 4-chlorobenzaldehyde was determined to be 2.2 mg/L based on measured concentrations.
Executive summary:

The acute toxicity of 4-chlorobenzaldehyde to fathead minnow (Pimephales promelas), was determined in an aerated, semi static, 96-hour test. Treatments consisted of a dilution water control and the nominal concentrations of 2.6, 4.3, 7.2, 12 and 20 mg/L.

Based on the test results, the 96-hour LC50for fathead minnow (Pimephales promelas

for 4-chlorobenzaldehyde was determined to be 2.2 mg/L based on measured concentrations.