Tall oil, reaction products with ethanolamine

Adsorption / desorption

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 March 2015 to 13 March 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Radiolabelling:

no

Test temperature:

30 °C

Details on study design: HPLC method:

EQUIPMENT
- Test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

DETERMINATION OF DEAD TIME
- Method: The dead time was determined by measuring the retention time of formamide (purity: 99.94%) at 622 mg/L in methanol:water (55:45 v/v).

REFERENCE SUBSTANCES
- Solutions of reference standards (table 5.4.1/1) were prepared in methanol.

PREPARATION OF THE SAMPLE SOLUTION
- Test item (0.1038 g) was diluted to 10 mL with methanol.

DETERMINATION OF RETENTION TIMES
- The sample, dead time and reference standard solutions were injected in duplicate using the following high performance liquid chromatography (HPLC) parameters:
HPLC system : Agilent Technologies 1200, incorporating workstation and autosampler
Detectors: Agilent variable wavelength detector (VWD) and Polymer Laboratories evaporative light scattering detector 2100 (ELSD)
Column : Water XSelect CN 5 µm (150 x 4.6 mm id)
Column Temperature : 30 °C
Flow rate : 1.0 mL/min
Mobil phase : methanol: purified water (55:45 v/v)
pH of mobile phase : 6.5
Injection volume : 10 μL
UV detector wavelength (dead time and reference standards): 210 nm
ELSD parameters (sample): nebuliser temperature: 40 °C; evaporator temperature: 80 °C; gas flow: 1.0 L/min

The mobile phase was ramped to 100% propan-2-ol shortly after the elution of the last reference standard to elute the highly retained test item components. This was carried out for the sample and sample blank injections only.

EVALUATION
- Calculation of capacity factors k':
The capacity factors were determined using the following equation:
k’ = (tr– t0)/ t0
where:
k’: capacity factor; tr: retention time (min); t0: dead time (min)

Construction of the calibration curve:
A correlation of logo k’ versus Log10Koc of the calibration standards was plotted using linear regression. The capacity factor (k’) for the reference standards was calculated from the retention time data of the dead time and reference standard solutions. Log10Koc values of the reference standards are those quoted in OECD 121.

- Determination of the log Koc value:
Adsorption Coefficient:
Log10Koc = (Log10k’-A) /B

Koc: adsorption coefficient
k’: capacity factor
A: intercept of the calibration curve
B: slope of the calibration curve

Type:

Koc

Remarks on result:

other: >4.27 x 10E05

Type:

log Koc

Value:

> 5.63

Temp.:

30 °C

Details on results (HPLC method):

- Typical chromatograms are attached.
- Retention times of the dead time and the retention times, capacity factors and log10 Koc values for the reference standards are shown in Tables 3.2 and 3.3 (below).
- The calibration curve is shown in Figure 3.1 (attached).
- The retention times, capacity factor and log10 Koc value determined for the sample are shown in Table 3.4 (below).

Table 3.2: Retention times of the dead time

Dead Time	Retention Time (min)		Mean Retention Time (min)
	Injection 1	Injection 2
Formamide	1.919	1.921	1.920

 

Table 3.3: Retention times, capacity factors andLog10 Koc values for the reference standards

Standard	Retention Time (min)		Mean Retention Time (min)	Capacity Factor (k’)	Log10k’	Log10Koc
	Injection 1	Injection 2
Acetanilide	2.625	2.625	2.625	0.367	-0.435	1.25
Atrazine	3.615	3.600	3.607	0.879	-5.62 x 10^-2	1.81
Isoproturon	3.840	3.833	3.837	0.998	-8.24 x 10^-4	1.86
Triadimenol	5.129	5.126	5.127	1.67	0.223	2.40
Linuron	5.213	5.209	5.211	1.71	0.234	2.59
Naphthalene	4.534	4.532	4.533	1.36	0.134	2.75
Endosulfan-diol	6.376	6.378	6.377	2.32	0.366	3.02
Fenthion	7.647	7.649	7.648	2.98	0.475	3.31
α-Endosulfan	10.762	10.752	10.757	4.60	0.663	4.09
Diclofop-methyl	11.584	11.589	11.587	5.03	0.702	4.20
Phenanthrene	8.338	8.333	8.336	3.34	0.524	4.09
DDT	22.630	22.632	22.631	10.8	1.03	5.63

 

Table 3.4: Adsorption coefficient of sample

Injection	Retention Time (min)	Capacity Factor (k’)	Log10k’	Log10Koc	Mean Log10Koc	Adsorption Coefficient
1	≥23.223	>10.8	>1.03	>5.63	>5.63	>4.27 x 10^5
2	≥23.333	>10.8	>1.03	>5.63

 

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test item was assessed in accordance with OECD Guideline 121 and has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.

Executive summary:

GUIDELINE

The determination was carried out using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001, and Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008.

METHOD

The test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

RESULTS

The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63.

Reference 2

Endpoint:

adsorption / desorption: screening

Remarks:

Further information on adsorption/ desorption

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance and its relevant degradation products decompose rapidly

Description of key information

 The adsorption coefficient of the test item was assessed in accordance with OECD Guideline 121 and has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63. 

Additional information

Adsorption / desorption

GUIDELINE

The determination was carried out using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001, and Method C.19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008.

METHOD

The test utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

RESULTS

The adsorption coefficient of the test item has been determined to be greater than 4.27 x 10E05 with log10 Koc > 5.63. Further information on adsorption / desorptionThe test item is not classified as hazardous to the environment and has been demonstrated to be readily biodegradable. As a result, and in accordance with REACH Annex IX, Section 9.3.3, Column 2, further information on adsorption/desorption is not considered relevant or necessary.