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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test item attained 43 % biodegradation after 28 days and 49 % degradation after 36 days and was determined to be not readily biodegradable (OECD 301 B, EU Method C.4 -C and OCSPP 835.311).

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

GUIDELINE

A study was performed to assess ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (m)).

 

METHODS

The test item, at a concentration of 10 mg carbon/L, was exposed to activated sewage sludge micro-organisms with mineral medium in sealed culture vessels in the dark at temperatures of between 20 and 25 °C for 35 days. At the request of the Sponsor the study was extended from 28 Days to 35 Days. The biodegradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.

 

RESULTS

The test item attained 43 % biodegradation after 28 days and 49 % degradation after 36 days andwas determined to be not readily biodegradable under the strict terms and conditions of OECD Guideline No. 301 B.