Registration Dossier
Registration Dossier
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EC number: 206-033-9 | CAS number: 294-62-2
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- approx. 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity
- Author:
- Sparnins VL, Lam LKT and Wattenberg LW
- Year:
- 1 984
- Bibliographic source:
- Biochem. Biophys. Res. Commun. 120, 2, 637-640
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Deviations:
- not applicable
- Principles of method if other than guideline:
- see information in sections "Test animals", "Administration/exposure" and "Examinations"
- GLP compliance:
- no
- Type of method:
- in vivo
- Endpoint addressed:
- basic toxicokinetics
Test material
- Reference substance name:
- Cyclododecane
- EC Number:
- 206-033-9
- EC Name:
- Cyclododecane
- Cas Number:
- 294-62-2
- Molecular formula:
- C12H24
- IUPAC Name:
- cyclododecane
- Details on test material:
- Cyclododecane from Dupont, purity not reported
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ORGANISM
- Female ICR/Ha mice
- Source: Madison, WI colony of Harlan-Sprague-Dawley, Indianapolis
- Age: 7 weeks
- Number: 5-10 animals per group
- Control: diet
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- other: diet
- Details on exposure:
- TREATMENT
- 30 or 50 µmol/g in diet for 2 weeks - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 2 weeks
- Frequency of treatment:
- daily
- Post exposure period:
- no
Doses / concentrations
- Remarks:
- Doses / Concentrations:
30 or 50 µmol/g diet
Basis:
nominal in diet
- No. of animals per sex per dose:
- 5 - 10 females per dose
- Control animals:
- yes, concurrent no treatment
- Details on study design:
- no further details
Examinations
- Examinations:
- EXAMINATIONS
- after exposure removal and homogenization of liver, forestomach, and mucosa from small bowel
- GST activity determination in the supernatant using 1-chloro-2,4-dinitrobenzene as substrate - Positive control:
- no data
Results and discussion
- Details on results:
- see below (section "Remarks on results including tables and figures")
Any other information on results incl. tables
None of the compounds elicited increased GST activity in the forestomach. C6 ring compounds showed no significant effect.
C12 ring compounds were more effective than C8 ring compounds with a decrease in activity in the order:
unsaturated > epoxide > alcohol > saturated.
Among the C12 ring compounds tested, cyclododecane induced the slightest increase of the GST activity in liver and small bowel
mucosa:
-----------------------------------------------------------
Test Compound Liver Small Bowel Mucosa
Specific Activity Specific Activity
-----------------------------------------------------------
None
1.39 +- 0.05 0.27 +- 0.01
Cyclododecane 1.84 +- 0.13 0.53 +- 0.04
Applicant's summary and conclusion
- Conclusions:
- Cyclododecane induced a slight increase of the GST activity in liver and small bowel mucosa.
- Executive summary:
Glutathione S-transferase is considered as a major detoxification system which catalyzes conjugation of electrophilic compounds,
e.g. chemical carcinogens, to glutathione. The effects of cyclic 12-, 8- and 6-carbon compounds (incl. cyclododecane) on the
glutathione S-transferase (E.C 2.5.1.18) activity in the liver, intestinal mucosa and the forestomach of female ICR/Ha mice were
investigated.
None of the compounds elicited increased GST activity in the forestomach. C6 ring compounds showed no significant effect.
C12 ring compounds were more effective than C8 ring compounds with a decrease in activity in the order:
unsaturated > epoxide > alcohol > saturated.
Among the C12 ring compounds tested, cyclododecane induced the slightest increase of the GST activity in liver and small bowelmucosa.
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