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EC number: 299-682-2 | CAS number: 93893-89-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Auto flammability
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- Additional physico-chemical properties of nanomaterials
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-03-02 to 2007-03-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Version / remarks:
- 2002
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 3-methyl-5-phenylpent-2-enenitrile
- EC Number:
- 299-682-2
- EC Name:
- 3-methyl-5-phenylpent-2-enenitrile
- Cas Number:
- 93893-89-1
- Molecular formula:
- C12 H13 N
- IUPAC Name:
- (2E)-3-methyl-5-phenylpent-2-enenitrile
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 8-12 weeks
- Weight at study initiation: 15-23 g
- Housing: individually
- Diet: certified rat and mouse diet, ad libitum
- Water: tap water ad libitum
- Acclimation period: at least five days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-25
- Humidity (%): 30-70
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 1, 0.5, 0.25 %
- No. of animals per dose:
- 4
- Details on study design:
- RANGE FINDING TESTS:
- Irritation: Seven mice were treated daily by application of 25 µL of the undiluted test material and concentrations of 50, 25, 10, 5, 2.5 and 1 %. Severe signs of systemic toxicity were observed in all dose groups except the 1 % dose group. The dose level selected for the main test was therefore 1 %.
MAIN STUDY
TREATMENT PREPARATION AND ADMINISTRATION:
Groups of four mice per concentration were treated by daily application of 25 µL to the dorsal surface of each ear for three consecutive days. Administration was carried out using an automatic micropipette and spread over the dorsal surface of the ear using the tip of the pipette. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- The proliferation response of lymph node cells was expressed as the number of radioactive disintegrations per minute per lymph node (disintegrations per minute/node) and as the ratio of 3HTdR incorporation into lymph node cells of test nodes relative to that recorded for the control nodes (Stimulation Index).
The EC3 value is the concentration of test material expected to cause a 3 fold increase in HTdR incorporation.
The equation used for the calculation of EC3 is:
EC3 = c + [(3-d/b-d) x (a-c)]
a = lowest concentration giving stimulation index >3
b = actual stimulation index caused by a
c = highest concentration failing to produce a stimulation index of 3
d = actual stimulation index caused by c
Results and discussion
- Positive control results:
- The stimulation index (expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporation of the vehicle control group) of a 5 % (v/v) concentration in acetone/ olive oil 4:1 was 2.5, the SI of a 10 % concentration was 4.03 and the SI of a 25 % concentration was 9.13. Therefore, the positive control substance was considerd to be a sensitiser.
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- EC3
- Value:
- 0.77
- Test group / Remarks:
- in %
- Parameter:
- SI
- Value:
- 3.75
- Test group / Remarks:
- Test substance concentration 1 %
- Parameter:
- SI
- Value:
- 2.16
- Test group / Remarks:
- Test substance concentration 0.5 %
- Parameter:
- SI
- Value:
- 2.11
- Test group / Remarks:
- Test substance concentration 0.25 %
Any other information on results incl. tables
Table 1: Disintegrations per Minute, Disintegrations per Minute/Node and Stimulation Index
Concentration (% v/v) in acetone/olive oil 4:1 |
DPM |
DPM/Node |
Stimulation Index |
Result |
Vehicle |
7740.67 |
967.58 |
NA |
NA |
0.25 |
16328.71 |
2041.09 |
2.11 |
Negative |
0.5 |
16749.20 |
2093.65 |
2.16 |
Negative |
1 |
29046.08 |
3630.76 |
3.75 |
Positive |
An EC3 value of 0.77% was calculated.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1A (indication of significant skin sensitising potential) based on GHS criteria
- Conclusions:
- The test substance was found to be skin-sensitising.
- Executive summary:
A study was performed to assess the skin sensitization potential of the test material in the CBA/Ca strain mouse following topical application to the dorsal surface of the ear. The study was performed according to OECD Guideline 429 and GLP. Following a preliminary screening test, three groups, each of four animals, were treated with 50 µL (25 (µL per ear) of the test material as a solution in acetone/olive oil 4:1 at concentrations of 1 %, 0.5 % or 0.25 % v/v. A further group of four animals was treated with acetone/olive oil 4:1 alone. The stimulation indices of the concentrations 0.25, 0.5 and 1 % were 2.11, 2.16 and 3.75, respectively. An EC3 value of 0.77 % was calculated. Therefore, the test result of the substance at a concentration of 1 % was considered positive and the test substance was found to be a skin sensitiser (Cat. 1A).
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