Reaction mass of disodium [2,2'-(imino-κN)dibutanedioato-κ2O1,O4(4-)]calcium(2-), sodium chloride and aspartic acid disodium salt

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

GLP compliance:

not specified

Type of method:

flask method

Water solubility:

695.83 mg/L

Conc. based on:

test mat.

Incubation duration:

>= 24 - <= 72 h

Temp.:

20 °C

pH:

8

Details on results:

The absorbance for individual samples was measured. The apparatus read out the level of concentration automatically .
The contents of chelate in the tested samples was calculated using the following formula:
X=c*25*v1/m*v2, where:
X - contents of chelate, [mg/g];
c - concentration of chelate read from the relevant calibration curve, [mg/ml]
25 - volume of flask with direct solution, [ml]
m - weighed amount of sample; [g]
v1- volume of flask from dilution, [ml]
v2 - volume of the direct solution taken for dilution, [ml]

Result for caIDHA in aqueos solution.

CaIDHA in aqueous solution	Result after 24h	Result after 48h	Result after 72h
	0.0243	0.0222	0.0203
Solubility [g/dm3]	759.38	963.75	634.38
Average	695.83
Difference between two most recent measurements	9.4%

Conclusions:

Solubility of CaIDHA chelate in water in temperature 20 ± 0.5°C is: 695.83 mg/l.

Executive summary:

The subject of the task was the execution of study of solubility in water for chelate CaIDHA according to the OECD Test Guideline A6 (OECD 105). The method relied on the measurement of concentration of chelate (content of chelate) in solution tested with spectrophotometer based on calibration curves (prepared for water).

The following analysis was performed:

1.  Initial study (sequential procedure)

2.  Measurement of pH for samples using test-papers in water,

3.  Determination of mass concentration of substance tested in water using the shake - flask method:

a)  UV spectrum

b)  Calibration curve

c)  Measurement of concentration (contents) of the chelate with spectrophotometric method after 24h, 48h as well as 72h.

All obtained results for  meet the assumption of the OECD Test Guideline A6 (OECD 105): The result of determination in at least two last vessels (measurements after 48h and 72h) is not different by more than 15%. Solubility of CaIDHA chelate in water  in temperature 20 ± 0.5°C is: 695.83 mg/l.

Description of key information

All obtained results for meet the assumption of theOECD Test Guideline A6 (OECD 105):The result of determination in at least two last vessels (measurements after 48h and 72h) is not different by more than 15%. Solubility of CaIDHA chelate in water  in temperature 20 ± 0.5°C is: 695.83 mg/l

Key value for chemical safety assessment

Water solubility:

695.83 mg/L

at the temperature of:

20 °C