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EC number: 257-840-8 | CAS number: 52314-67-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from safety assessment report
Data source
Referenceopen allclose all
- Reference Type:
- review article or handbook
- Title:
- LETTER FROM PHILLIPS PETROLEUM CO TO US EPA SUBMITTING UNPUBLISHED STUDIES ON CYCLOHEXANE WITH ATTACHMENTS
- Author:
- NTRL
- Year:
- 1 986
- Bibliographic source:
- NTRL REPORT- OTS0527456; January15, 1986
- Reference Type:
- secondary source
- Title:
- European Union Risk Assessment Report -CYCLOHEXANE
- Author:
- European Communities
- Year:
- 2 004
- Bibliographic source:
- European Union Risk Assessment Report -CYCLOHEXANE ; European Communities Final Report, 2004.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Washed and unwashed primary eye irritation studies of chemical Cyclohexane (CAS No: 110-82-7) was performed in six male and female rabbits to assess its eye irritation potential.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Cyclohexane
- Cas Number:
- 110-82-7
- Molecular formula:
- C6H12
- IUPAC Name:
- Cyclohexane
- Test material form:
- liquid
- Details on test material:
- - Name of test material (as cited in study report):Cyclohexane
- Molecular formula: C6H12
- Molecular weight: 84.16g/mole
- Substance type: organic
- Physical state: liquid
- InChI: 1S/C6H12/c1-2-4-6-5-3-1/h1-6H2
- Smiles: C1CCCCC1
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report):Cyclohexane
- Molecular formula: C6H12
- Molecular weight: 84.16g/mole
- Substance type: organic
- Physical state: liquid
- InChI: 1S/C6H12/c1-2-4-6-5-3-1/h1-6H2
- Smiles: C1CCCCC1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- not specified
- Details on test animals or tissues and environmental conditions:
- No data available
Test system
- Vehicle:
- not specified
- Controls:
- not specified
- Amount / concentration applied:
- No data available
- Duration of treatment / exposure:
- 7 days
- Observation period (in vivo):
- 7 days
- Duration of post- treatment incubation (in vitro):
- No data available
- Number of animals or in vitro replicates:
- 6/group (3/sex)
- Details on study design:
- Details on study design
TEST SITE
- Time after start of exposure: 24 hours
-SCORING SYSTEM: according to Draize scoring system
Results and discussion
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 7 d
- Score:
- 0
- Max. score:
- 110
- Reversibility:
- fully reversible within: 24 hours
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- In the unwashed study, at one hour post instillation, corneal opacity, involving up to 25% of the cornea, was noted in one rabbit and iritis was noted in another rabbit. Conjunctival redness was noted in five rabbits with conjunctival chemosis in one rabbit. All ocular lesions had cleared within 24 hours and no conjunctival discharge was noted in any of the six animals.
In the washed study, Conjunctival redness (vessels injected above normal) was noted in four rabbits at one hour postinstillation and had cleared by 24 hours. No corneal opacity, iritis, conjunctival chemosis, or discharge 'was noted in any of the six rabbits.
Any other information on results incl. tables
Table 1: Eye Irritation Score Ranges and Means d with Standard Deviations washed Primary Eye Irritation Study of Cyclohexane in Rabbits |
||||||
OBSERVATION |
OBSERVATION INTERVALS |
|||||
HOURS |
DAYS |
|||||
1 |
24 |
48 |
72 |
4 |
7 |
|
Cornea |
|
|
|
|
|
|
Mean |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
- |
- |
- |
- |
- |
- |
|
||||||
Iris |
|
|
|
|
|
|
Mean |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
- |
- |
- |
- |
- |
- |
|
||||||
Conjunctivae |
|
|
|
|
|
|
Mean |
1.3 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
1.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
0-2 |
- |
- |
- |
- |
- |
|
||||||
Total score |
|
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Mean |
1..3 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
1.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
0-2 |
- |
- |
- |
- |
- |
*Mean based on six observations |
Table 2: Eye Irritation Score Ranges And Means D With Standard Deviations Unwashed Primary Eye Irritation Study Of Cyclohexane In Rabbits |
||||||
OBSERVATION |
OBSERVATION INTERVALS |
|||||
HOURS |
DAYS |
|||||
1 |
24 |
48 |
72 |
4 |
7 |
|
Cornea |
|
|
|
|
|
|
Mean |
0.8 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
2.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
0-5 |
- |
- |
- |
- |
- |
|
||||||
Iris |
|
|
|
|
|
|
Mean |
0.8 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
2.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
0-5 |
- |
- |
- |
- |
- |
|
||||||
Conjunctivae |
|
|
|
|
|
|
Mean |
2.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
1.3 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
0-4 |
- |
- |
- |
- |
- |
|
||||||
Total score |
|
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Mean |
3.7 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
S.D |
2.9 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
Range |
0-7 |
- |
- |
- |
- |
- |
*Mean based on six observations |
Applicant's summary and conclusion
- Interpretation of results:
- other: Not irritating
- Conclusions:
- The test material was considered to be not irritating when installed into the eye of each rabbits in both the washed and unwashed primary eye irritation studies.
- Executive summary:
Washed and unwashed primary eye irritation studies of chemical Cyclohexane (CAS No: 110-82-7) was performed in six male and female rabbits to assess its eye irritation potential.
The chemical was installed into the one of each animal and observations were made 24 hours after treatment.
In the unwashed study, at one hour post instillation, corneal opacity, involving up to 25% of the cornea, was noted in one rabbit and iritis was noted in another rabbit. Conjunctival redness was noted in five rabbits with conjunctival chemosis in one rabbit. All ocular lesions had cleared within 24 hours and no conjunctival discharge was noted in any of the six animals.
In the washed study, Conjunctival redness (vessels injected above normal) was noted in four rabbits at one hour postinstillation and had cleared by 24 hours. No corneal opacity, iritis, conjunctival chemosis, or discharge 'was noted in any of the six rabbits.
Since all the observed effects were cleared within 24 hours, the chemical Cyclohexane (CAS No: 110-82-7) was considered to be non-irritant to the eyes of rabbits.
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