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EC number: 236-752-3 | CAS number: 13474-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 Feb 2015 to 25 Mar 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 9439 Water Quality - Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds - Method by analysis of released carbon dioxide.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, Germany.
Test material
- Reference substance name:
- 3-methyl-1-vinyl-1H-imidazolium chloride
- EC Number:
- 236-752-3
- EC Name:
- 3-methyl-1-vinyl-1H-imidazolium chloride
- Cas Number:
- 13474-25-4
- Molecular formula:
- C6H9N2.Cl
- IUPAC Name:
- 1-ethenyl-3-methyl-2,3-dihydro-1H-imidazol-1-ium chloride
Constituent 1
- Specific details on test material used for the study:
- - Name of the test material (as cited in study report): 3-Methyl-1-vinyl-1H-imidazolium chloride
- Test substance No.: 14/0365-1
- Batch identification: Mischprobe B 407 / 408 vom 23.08.14, 14001329T0 - 14001629T0
- Identity: Confirmed by several spectroscopic methods
- Purity: 97.7 area-% (203 nm) and 98.2 area-% (220 nm), HPLC Fingerprint and identification of constituents by MS. Main component: 58.3g/100g.
- Homogeneity: Homogeneous
- Date of production: August 2014
- Physical state / apperance: liquid, brownish yellow, clear
- Expiry date: August 2015.
- Storage conditions: room temperature
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge from municipal waste water treatment plant Mannheim, Germany. The inoculum was collected on 20 February 2015 from the aeration tank of the plant. A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon in the blank controls the activated sludge was aerated with carbon dioxide free air for about 96 hours at 22 ± 2° C. At the day of exposure the suspension was washed one time with drinking water. Therefore the aeration was stopped and the sludge was allowed to settle. After settling the supernatant was discarded and the remaining sludge suspension was filled up with drinking water and the concentration oft the sludge was adjusted to 6.0 g/L dry weight.
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 25.6 mg/L
- Based on:
- test mat.
- Initial conc.:
- 7.5 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of mineral medium: Guideline solutions A-D, dissolved in 1 L water.
1. Solution A: KH2PO4: 8.50 g; K2HPO4: 21.75 g; Na2HPO4 × 2 H2O: 33.40 g; NH4Cl: 0.50 g
2. Solubiton B: CaCl2 × 2 H2O: 36.40 g
3. Solution C: MgSO4 × 7 H2O: 22.50 g
4. Solution D: FeCl3 × 6 H2O: 0.25 g
- Test temperature: 22 ± 2° C
- pH: adjusted to 7.4 ± 0.2 if necessary; pH-values at the end of exposure were 7.0 - 7.2
- Continuous darkness: no
- Stirring: incubation bottles were stirred on magnetic stirrers;
- Aeration: Yes, performed with carbon dioxide free air at a flow of approximately 800 mL per hour
PREPARATION
- Addition of medium: 15 mL solution A, 1.5 mL solution B, 1.5 mL solution C and 1.5 mL solution D
- Preparation of test vessels: required amounts of the test substance aliquots for the scheduled test concentration of 7.5 mg/L TOC were weight onto small glass plates (microscope cover slips) and completely added with the glass plates to the vessels of test substance assays and to the vessel of inhibition control. These vessels were stirred for about 30 minutes to ensure the dissolving of the test substance in the mineral medium. Finally enough reference substance stock solution was added to reach 20 mg TOC/L in the reference substance assay and 7.5 mg TOC/L in the inhibition control.
- Addition of sludge: Aliquots of activated sludge suspension were added to the test vessels to obtain an activated sludge concentration of 30 mg/L dry weight.
TEST SYSTEM
The Carbon Dioxide Evolution Test was performed in 2 L incubation bottles filled up to a volume of 1.5 L. The bottles were connected to two serial scrubbing bottles (total volume 250 mL) filled with 100 mL 0.05 mol sodium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. The TIC-value of the freshly prepared sodium hydroxide solution was determined and considered by the calculation of biogenic produced carbon dioxide amount.
SAMPLING
- TIC: Usually twice a week the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined and used for the calculation of the produced carbon dioxide. After each sampling the second trap was moved forward and the new trap with fresh sodium hydroxide solution was placed into the second position. Each trap was analyzed separately. At the begin and at the end of exposure, dissolved organic carbon (DOC) samples were taken from the test vessels of the blank control and the reference substance control.
- DOC: For determination of the decrease of dissolved organic carbon (DOC) samples were taken from the test vessels of the blank control and from the test vessel of the reference substance control and the DOC content was determined after centrifugation (approx. 15 minutes at 4000 rpm). See 'Any other information on materials and methods incl. tables' as to why no DOC measurements from the test assays were performed.
- pH: At the end of exposure, the pH values were measured in each test vessel.
CONTROL AND BLANK SYSTEM
- 2 blank control assays
- 1 inhibition control test assay
- 1 reference substance assay
Reference substance
- Reference substance:
- aniline
- Remarks:
- TOC concentration of the ref. substance: 20 mg/L(nominal value)
Results and discussion
- Test performance:
- - Amount of produced CO2 in the blank controls at the end of exposure (mean value): 39.1 mg/L
- Deviation of the degree of biodegradation of the test substance in the plateau phase should was <20%
- The degree of biodegradation of the reference substance was >60% CO2/ThCO2 after 14 days
- The degree of biodegradation in the inhibition control was >25% CO2/ThCO2 after 14 days
- The amount of produced CO2 in the inoculum blank (“blank controls”) at the end of exposure (mean value) was <70 mg/L
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 20 - <= 30
- Sampling time:
- 28 d
- Details on results:
- Duration of the adaptation phase was 23 days. The biodegradation was 23% CO2/ThCO2 (mean value) at day 28. The required pass level of 60% for ready biodegradability within a ten days window was thus not reached. For more details, see ‘Any other information on results incl. tables’.
BOD5 / COD results
- Results with reference substance:
- The biodegradation of the reference substance after 14 days was 68% (CO2/ThCO2).
Any other information on results incl. tables
Table: degree of biodegradation [% Co2/ThCO2]
Test duration [days] |
RS |
IH |
TS1 |
TS2 |
TS mv |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
-1 |
2 |
1 |
3 |
1 |
2 |
-5 |
5 |
0 |
6 |
14 |
14 |
-9 |
7 |
-1 |
10 |
50 |
22 |
-13 |
11 |
-1 |
14 |
68 |
27 |
0 |
15 |
8 |
17 |
78 |
26 |
1 |
4 |
3 |
20 |
85 |
28 |
-11 |
4 |
-4 |
22 |
89 |
32 |
9 |
6 |
8 |
27 |
98 |
41 |
22 |
16 |
19 |
28 |
101 |
47 |
24 |
22 |
23 |
RS: reference substance assay
IH: inhibition control assay
TS: test substance assay
mv: mean value
Table: Measured data of total inorganic carbon TIC [mg/L]
Day |
|
BC NaOH |
BC1 |
BC2 |
RS |
IH |
TS1 |
TS2 |
0 |
a |
0.7 |
- |
- |
- |
- |
- |
- |
b |
0.9 |
- |
- |
- |
- |
- |
- |
|
1 |
a |
0.7 |
4.9 |
3.1 |
5.3 |
4.5 |
2.6 |
6.3 |
b |
0.6 |
5 |
3.3 |
5.4 |
4.5 |
2.7 |
6.4 |
|
3 |
a |
1 |
10.2 |
7.2 |
10.8 |
13.6 |
4.7 |
11.8 |
b |
0.9 |
10 |
7.2 |
10.7 |
13.8 |
4.2 |
11.8 |
|
6 |
a |
0.5 |
19.3 |
15.4 |
56.7 |
44.2 |
12.3 |
20.3 |
b |
0.6 |
19.2 |
15.1 |
56.9 |
44.1 |
12.3 |
20.4 |
|
10 |
a |
0.9 |
21.1 |
12.2 |
122 |
34.6 |
12.8 |
21 |
b |
0.9 |
21 |
12.4 |
124 |
34 |
12.9 |
21.2 |
|
14 |
a |
0.8 |
19.4 |
22.5 |
76.5 |
32.7 |
34.4 |
26 |
b |
0.7 |
19.2 |
22.4 |
77.1 |
32.8 |
35.8 |
25.9 |
|
17 |
a |
0.7 |
23.9 |
33.4 |
58.1 |
26.1 |
30.7 |
15.5 |
b |
0.7 |
24.1 |
33.5 |
58 |
25.9 |
30.7 |
16 |
|
20 |
a |
0.6 |
22.8 |
20.1 |
42.5 |
27 |
6.9 |
21.4 |
b |
0.6 |
23.1 |
20.3 |
42.5 |
26.4 |
7.2 |
21.5 |
|
22 |
a |
0.5 |
11 |
10.9 |
21.9 |
20 |
34 |
12.9 |
b |
0.4 |
11 |
11 |
21.6 |
19.5 |
33.6 |
13.2 |
|
27 |
a |
0.7 |
15.9 |
12.7 |
40.6 |
33.7 |
28.8 |
26.4 |
b |
0.8 |
15.5 |
12.9 |
40.9 |
33.6 |
28.4 |
26.4 |
|
28 |
a |
0.5 |
6.4 |
6.2 |
13 |
10.3 |
7.9 |
8.7 |
b |
0.6 |
6.4 |
6.1 |
12.5 |
10.4 |
8 |
8.7 |
|
flask 1 29 |
a |
- |
15.6 |
14.3 |
18.4 |
23.1 |
16.6 |
18.8 |
b |
- |
15.5 |
14.1 |
18.6 |
23 |
16.3 |
18.9 |
|
flask 2 29 |
a |
- |
4.2 |
3.5 |
4.3 |
4.7 |
3.4 |
3.3 |
b |
- |
4.2 |
3.5 |
4.1 |
4.7 |
3.4 |
3.4 |
The NaOH blank value is determined on filling the absorption vessels.
The measured values, for instance applies on day 0 for the first and the second sampling.
The NaOH blank value was subtracted from each test vessel.
a/b = single values of TIC-measurement
Table: Produced carbon dioxide amount in the test vessels
[mg CO2/test vessel] |
mg CO2 added up after subtraction of the mean value of the blank controls. |
||||||||
Duration of exposure [days] |
BC mv |
RS |
IH |
TS1 |
TS2 |
RS |
IH |
TS1 |
TS2 |
1 |
1.2 |
1.7 |
1.4 |
0.7 |
2 |
0.5 |
0.2 |
-0.5 |
0.8 |
3 |
2.9 |
3.7 |
4.7 |
1.3 |
4 |
1.3 |
2 |
-2.1 |
1.9 |
6 |
6.1 |
20.6 |
16 |
4.3 |
7.2 |
15.8 |
11.9 |
-3.9 |
3 |
10 |
5.8 |
44.9 |
12.2 |
4.4 |
7.4 |
54.9 |
18.3 |
-5.3 |
4.6 |
14 |
7.5 |
28 |
11.8 |
12.7 |
9.3 |
75.4 |
22.6 |
-0.1 |
6.4 |
17 |
10.2 |
21 |
9.2 |
10.9 |
5.4 |
86.2 |
21.6 |
0.6 |
1.6 |
20 |
7.6 |
15.3 |
9.5 |
2.3 |
7.6 |
93.9 |
23.5 |
-4.7 |
1.6 |
22 |
3.8 |
7.7 |
7 |
12.1 |
4.5 |
97.8 |
26.7 |
3.6 |
2.3 |
27 |
5 |
14.7 |
12.1 |
10.3 |
9.5 |
107.5 |
33.8 |
8.9 |
6.8 |
28 |
2.1 |
4.5 |
3.6 |
2.8 |
3 |
111.4 |
38.6 |
10 |
9 |
29 |
6.4 |
7.9 |
9.7 |
6.8 |
7.7 |
|
|
|
|
Table: Measurement of Dissolved Organic Carbon
|
|
[mg/L DOC] |
|||||||
|
BC1 |
BC2 |
BC mv |
RS |
IH |
TS1 |
TS2 |
TS mv |
|
At begin of exposure |
a |
0.3 |
0.4 |
0.3 |
19.9 |
- |
- |
- |
|
b |
0.3 |
0.3 |
19.9 |
- |
- |
- |
|
||
At the end of exposure |
a |
1.7 |
1.3 |
1.6 |
1.5 |
- |
- |
- |
|
b |
1.8 |
1.6 |
1.6 |
- |
- |
- |
|||
Degradation of DOC [%] |
101 |
- |
- |
- |
- |
INHIBITION CONTROL
The degree of biodegradation in the inhibition control after 14 days was 27% CO2/ThCO2.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see 'Test performance'
- Interpretation of results:
- not readily biodegradable
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