Reaction mass of 3-phenyl-6-[(3-phenyl-3,4-dihydro-2H-1,3-benzoxazin-8-yl)methyl]-3,4-dihydro-2H-1,3-benzoxazine and 6,6'-methanediylbis(3-phenyl-3,4-dihydro-2H-1,3-benzoxazine)

Toxicity to aquatic algae and cyanobacteria

Description of key information

A study was performed to assess the effect of the test item on the growth of Pseudokirchneriella subcapitata. The test was performed according to the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria.

This data is coming from a read across ARALDITE MT 35600 (SOURCE) to ARALDITE 35700 (TARGET).

The test item is very low soluble therefore the test solutions was prepared as a Water Accommodated Fraction (WAF).

Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to a WAF of the test item, at a single nominal loading rate of 100 mg/L.

Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group.

Analysis of the test preparations at 0 and 72 hours showed measured test concentrations of less than the limit of quantification (LOQ) of the analytical method employed were obtained which was determined to be 0.031 mg/L.

EL₅₀values > 100 mg/L loading rate WAF. 

NOEL= 100 mg/L loading rate WAF.

According to the results, the test item is not toxic for Daphnia magna under the conditions of the test.

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L