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EC number: 224-030-0 | CAS number: 4170-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 006
Materials and methods
- Principles of method if other than guideline:
- sperm head abnormality assay
- GLP compliance:
- not specified
- Type of method:
- in vivo
Test material
- Reference substance name:
- Crotonaldehyde
- EC Number:
- 224-030-0
- EC Name:
- Crotonaldehyde
- Cas Number:
- 4170-30-3
- Molecular formula:
- C4H6O
- IUPAC Name:
- but-2-enal
- Details on test material:
- no data
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- laboratory bred swiss albino mice were obtained from central Drug Research institute (Lucknow) and maintained in animal house of the laboratory conducing the study.
6 to 8 weeks old
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- olive oil
- Details on exposure:
- none
- Analytical verification of doses or concentrations:
- not specified
- Details on analytical verification of doses or concentrations:
- none
- Duration of treatment / exposure:
- 1 IP dose at 8, 16 or 32 ul/kg bw
animals were sacrificed 1, 3 or 5 weeks after treatment. - Frequency of treatment:
- once
- Duration of test:
- none
Doses / concentrations
- Remarks:
- Doses / Concentrations:
8, 16, or 32 ul/kg bw
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5 male per dose and time (15 per dose total)
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- none
- Statistics:
- student's t test
Results and discussion
Effect levels
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- 8 other: ul/kg bw
- Sex:
- male
- Basis for effect level:
- other: no abnormal sperm morphology
- Dose descriptor:
- LOAEL
- Effect level:
- 16 other: ul/kg bw
- Sex:
- male
- Basis for effect level:
- other: abnormal sperm head morphology
Observed effects
Any other information on results incl. tables
Effect of crotonaldehyde treatment on the incidence of sperm abnormality in Swiss albine mice at different stages of spermatogenesis
Dose (ul/kg bw) |
Sampling Week |
Population of treated cells |
Number of abnormal sperm |
Mean (% +/- SE) |
Control |
1 |
Spermatozoa |
58 |
2.32 ± 0.89 |
8 |
1 |
85 |
3.4 ± 0.78 |
|
16 |
1 |
125 |
5.0 ± 0.68* |
|
32 |
1 |
186 |
7.44 ± 0.9* |
|
|
|
|
|
|
Control |
3 |
Spermatid |
63 |
2.57 ± 0.25 |
8 |
3 |
99 |
3.96 ± 0.48 |
|
16 |
3 |
139 |
5.56 ± 0.56* |
|
32 |
3 |
209 |
8.36 ± 0.95* |
|
|
|
|
|
|
Control |
5 |
Pre-leptotene spermatogonia |
56 |
2.24 ± 0.38 |
8 |
5 |
66 |
2.62 ± 0.45 |
|
16 |
5 |
72 |
2.88 ± 0.48 |
|
32 |
5 |
141 |
5.64 ± 0.58* |
* Significantly different from controls at P<0.05 in Student’s t test
Effect of crotonaldehyde treatment on the incidence of sperm abnormality in Swiss albine mice at different stages of spermatogenesis
Dose (ul/kg bw) |
Sampling Week |
Types and percentage of abnormal sperm heads |
|||
Short hook |
Without hook |
Giant amorphous |
Banana type |
||
|
|
|
|
|
|
Control |
1 |
0.48 |
0.6 |
0.24 |
1.0 |
8 |
1 |
0.88 |
1.68 |
0.48 |
0.36 |
16 |
1 |
1.04 |
2.16 |
0.88 |
0.92 |
32 |
1 |
1.28 |
1.68 |
2.2 |
2.28 |
|
|
|
|
|
|
Control |
3 |
0.6 |
0.88 |
0.56 |
0.48 |
8 |
3 |
0.92 |
1.08 |
0.72 |
1.24 |
16 |
3 |
1.4 |
1.92 |
1.24 |
1 |
32 |
3 |
1.68 |
2.6 |
1.48 |
2.6 |
|
|
|
|
|
|
Control |
5 |
0.6 |
0.8 |
0.2 |
0.64 |
8 |
5 |
0.52 |
0.88 |
0.4 |
0.84 |
16 |
5 |
0.48 |
1.08 |
0.56 |
0.76 |
32 |
5 |
1.12 |
1.6 |
1.4 |
1.52 |
Applicant's summary and conclusion
- Conclusions:
- Statistically significant increase in the percentage of abnormal sperm heads were recorded at 16 and 32 ul/kg bw at 3 weeks only and only at 32 ul/kg bw at 5 weeks.
- Executive summary:
After single IP injection of male swiss albino mice with crotonaldehyde (8, 16 or 32 ul/kg bw) or vehicle (olive oil at 0.2 ml/kg bw) examinations of the number and percentage of abnormal sperm and different types of abnormal sperm heads were made after 1, 3 and 5 weeks. Animals treated with crotonaldehyde at 8, 16 and 32 ul/kg bw demonstrated a dose related increase in the percentage of abnormal sperm heads. Statistically significant increase in the percentage of abnormal sperm heads were recorded at 16 and 32 ul/kg bw at 3 weeks only and only at 32 ul/kg bw at 5 weeks. The common types of sperm head abnormalities recorded were short hooked, without hook, giant amorphous and banana type.
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