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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to fish

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations. The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on daphnia magna was predicted for 2-amino-3,5-dimethyl benzene- 1-sulfonic acid (88-22-2). EC50 value was estimated to be 30.906mg/l for daphnia magna for 48hrs duration. Based on this value it can be concluded that the 2-amino-3,5-dimethylbenzene-1-sulfonic acid is considered to toxic to aquatic environment and classified as aquatic chronic 3 as per the criteria mentioned in CLP regulation. 

Toxicity to aquatic algae and cyanobacteria:

Based on the growth inhibition of green alga Chlorella vulgaris by the test chemical 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88-22-2), the EC50 was determine to be 50.584 mg/l.

Additional information

Summarized results of toxic effect of the chemical 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88-22-2) on the growth and other normal activities of aquatic lifes (invertebrates and algae), by the collection of data from various databases for target chemical, and RA chemicals for evaluating the effects of chemical on the basis of structure similarities.

 

Short term toxicity to fish

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations.

The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

 

Based on the various experimental data and predicted data for the target chemical2-amino-3,5-dimethylbenzene-1-sulfonic acid, study have been reviewed to determine the toxic nature of2-amino-3,5-dimethylbenzene-1-sulfonic acid on the growth of aquatic invertebrates. The studies are as mentioned below:

In the first weight of evidence study for the target chemical 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88 -22 -2) from QSAR,

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on daphnia magna was predicted for 2-amino-3,5-dimethyl benzene- 1-sulfonic acid (88-22-2). EC50 value was estimated to be 30.906mg/l for daphnia magna for 48hrs duration. Based on this value it can be concluded that the 2-amino-3,5-dimethylbenzene-1-sulfonic acid is considered to toxic to aquatic environment and classified as aquatic chronic 3 as per the criteria mentioned in CLP regulation. 

Similarly in the second weight of evidence study for the RA chemical 6-Aminotoluene-3-sulfonic acid (98-33-9), from the ABITEC lab report study was carried out. Determination of the inhibition of the mobility of daphnids was carried out with the substance 6-Aminotoluene-3-sulfonic acid according to OECD Guideline 202. The test substance was tested at the concentrations 0, 30, 45, 67, 100, 150 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, 6-Aminotoluene-3-sulfonic acid, in Daphnia magna was determined to be 74.9 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as chronic category 3 as per the CLP criteria.

 

Similarly in the third weight of evidence study for the RA chemical 6-Amino-m-toluenesulfonic acid (88-44-8), j-check 2016, Short term toxicity study of 6-Amino-m-toluenesulfonic acid (2-Amino-5-methyl -benzenesulfonic acid) on the growth of daphnia magna. Static method was used which was performed by using the standard OECD guideline. Control, vehicle control was also used having the 10 mg/l concentration. After the 48hrs of exposure the EC50 was >10 mg/l, but after 48hrs NOEC was 10 mg/l. Thus based on the EC50 value, 6-Amino-m-toluenesulfonic acid (2-Amino-5-methylbenzenesulfonic acid) was consider as toxic and can be consider to be classified in aquatic chronic 3 category as per the CLP classification criteria.

 

Thus based on the data for target chemical 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88-22-2) from various data bases, 2-amino-3,5-dimethylbenzene-1-sulfonic acid was consider as toxic. Since the test chemical is readily biodegradable in nature, chemical can be considered as non-toxic to aquatic organisms and can be classified to be 'Not classified' as per the CLP regulation criteria.

 

 

 

Toxicity to aquatic algae and cyanobacteria:

To evaluate toxicity of aquatic algae, various experimental studies were performed.Based on the experimental data aquired from various sources following is the discription:

In an first experimental study report,the study was designed to assess the toxic effects of the test compound 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88-22-2) on the green alga Chlorella vulgaris. Test was conducted in compliance with the OECD guideline 201 (Alga, Growth Inhibition Test).

Test was carried out in 100mL conical flasks which were carefully autoclaved and sterilized. The test solution in each of these test vessels was kept constant which is 60 ml so that a sufficient amount of head space was left. The test substance 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88-22-2) was prepared by adding 50 mg of test item in 250 ml of BBM to get the final concentration of 200mg/L. This stock solution was kept for stirring for 05 minutes to obtain a homogenous solution for the experiment. The test concentrations 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L were chosen according to the available data of the test item. The concentrations chosen were set up to the water solubility limit. The remaining test solutions were prepared by dilution from the above stock solution.For the assessment of algal growth, the test was conducted in replicates. The control flask was maintained in triplicates as recommended in the OECD guideline and the test concentration were selected in geometric series which were maintained in duplicates. To obtain a quantitative concentration-response relationship by regression analysis, a linearizing transformation of the response data into probit was performed. Using the same, effective concentration (EC) were determined.Algal growth was calculated daily by counting the cells microscopically with the help of haemocytometer. For microscopic observations the cultures were observed daily with the help of a microscope to verify a normal and healthy appearance of the algal culture and also to observe any abnormal appearance of the algae (as may be caused by the exposure of the test item). Apart from this, the cell count of each test vessel was also noted with the help of a microscope and haemocytometer. By spectrophotometer the absorbance values of each test vessel and control vessel was noted at 680nm.The BBM was taken as blank for both control and test vessels. The absorbance value of each vessel was in line with the average specific growth rate.As per OECD 201, the biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72 hr test period. This corresponds to a specific growth rate of 0.92 per day. Thus, the observed specific growth rate in the control cultures during the experiment was 0.358 per day. Secondly the mean coefficient of variation for section by section specific growth rates (days 0-1, 1-2 & 2-3, for 72 hr tests) in the control cultures must not exceed 35%. Thus, the observed mean coefficient of variation in the control cultures during the experiment was 33.42%. Thirdly the coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 10%. Thus, the observed coefficient of variation of average specific growth rates during the experiment in control cultures was 8.26%. Hence, the test is considered valid as per OECD guideline, 201.After 72 hours of exposure to test item 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88-22-2) to various nominal test concentrations, EC50 was determine to be 50.584 mg/l graphically and through probit analysis. Based on the EC50, it can be concluded that the chemical was hazardous and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

In an another experimental study report,aim of the study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution of 50 ml was prepared by dissolving in OECD growth medium / reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration were used.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.The median effective concentration (EC50) for the test substance in algae was determined to be 96.2 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Thus, based on the experimental studies, we can consider that the test chemical 2-amino-3,5-dimethylbenzene-1-sulfonic acid (88-22-2) is toxic. Since the test chemical is readily biodegradable in nature, chemical can be considered as non-toxic to aquatic organisms and can be classified to be 'Not classified' as per the CLP regulation criteria.