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Table 1: Summary of Hatching, Survival, Abnormalities, and Swim-Up from Hatching to Thinning
Hatching to Thinning
Meana, Measured Concentration (mg total fluorinated solids/L)
First Day of Swim-Up
Dilution Water Control
a Calculated as mean of replicate values, percent is based on mean values.
Table 2: Summary of Survival, Abnormalities, and Growth from Thinning to Test End
Meanb, Measured Concentration (mg total fluorinated solids/L)
Thinning to Test End
Growth of Surviving Fingerlings
Mean Standard Length ± Std. Dev. (cm)
Mean Wet Weight ± Std. Dev. (cm)
4.9 ± 0.3
1.527 ± 0.260
4.9 ± 0.2
1.488 ± 0.157
5.0 ± 0.2
1.565 ± 0.252
1.508 ± 0.197
4.8 ± 0.3
1.403 ± 0.269
1.494 ± 0.193
b Calculated as mean of replicate values, percent is based on mean values.
Based on mean, measured fish tissue results on days 0-45 and days 0-90 and mean, measured test solution concentrations from day 0-48 (due to routine test solution sampling) and day 0-90, bioconcentration factors ranged from 0.637 to 3.01 L/g and from 0.410 to 1.18 L/g, respectively.
The effect of the test substance on hatching, growth and survival of rainbow trout, Oncorhynchus mykiss, embryos, alevins, and fingerlings was assessed in a flow-through 90-day early life-stage toxicity test. Nominal test concentrations were 3.23, 6.46, 12.9, 25.5, and 51.0 mg/L test substance (corresponding to nominal total fluorinated solids concentrations of 0.95, 1.9, 3.8, 7.5, and 15 mg/L) and dilution water control. Measured test substance concentrations were 0.648, 1.21, 2.69, 5.86, and 13.4 mg total fluorinated solids/L; none detected in dilution water control. A total of 80 embryos were exposed per concentration (20 embryos per embryo cup, 2 cups per replicate, 2 replicates per concentration) at test start. On day 45, after swim-up had begun in the dilution water control, the fingerlings were thinned to a total of 30 fish per concentration (15 fish per replicate, 2 replicates per concentration). Observations were made for assessment of number of dead eggs, first and last day of hatching, first day of swim-up, survival and abnormalities from hatching to thinning, and survival and abnormalities from thinning to test end. Standard length and blotted wet weight of surviving fingerlings were determined at test end. The No Observed Effect Concentration (NOEC) for the test substance was 5.86 mg total fluorinated solids/L based on mean, measured total fluorinated solids concentrations and first and last day of hatching. The NOEC values for other test endpoints were 13.4 mg total fluorinated solids/L, the highest tested concentration. Day 45 and day 90 bioconcentration factors were less than or equal to 3.01 and 1.18, respectively, corresponding to log BCF values of less than or equal to 0.479 and 0.072, respectively.
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