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EC number: 426-730-3 | CAS number: 123439-82-7
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant, guideline study, available as unpublished report, fully adequate for assessment..
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Commission directive 87/302/EEC
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 123439-82-7
- Cas Number:
- 123439-82-7
- IUPAC Name:
- 123439-82-7
- Reference substance name:
- Tetrammine platinum (II) hydrogen carbonate
- IUPAC Name:
- Tetrammine platinum (II) hydrogen carbonate
- Test material form:
- solid
- Remarks:
- powder
- Details on test material:
- - Description: white powder
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - An amount of test material (4.0 g) was dissolved in water with the aid of ultrasonic disruption and the volume adjusted to 1 L to give a 4.0 g/L stock solution. Aliquots (15.6, 31.3, 62.5, 125 and 250 mL) of the 4.0 g/L stock solution were removed and dispersed with activated sewage sludge, synthetic sewage and water to give the final concentrantions of 125, 250, 500, 1000 and 2000 mg/L.
- The control was dechlorinated laboratory tap water.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: a mixed population of activated sewage sludge microorganisms
- Name and location of sewage treatment plant where inoculum was collected: 'Severn Trent Water Plc' at Belper, Derbyshire, UK
- Method of cultivation: not reported
- Preparation of inoculum for exposure: the activated sewage sludge sample was maintained on continuous aeration in the laboratory at a temperature of 21 °C. At 0 hours, 16 mL of synthetic sewage was diluted to 300 mL with water and 200 mL of inoculum were then added in a 500 mL conical flask. The mixture was aerated with compressed air via narrow bore glass tubes at a rate of approximately 0.5 - 1 L per minute. Thereafter, at 15-minute intervals the procedure was repeated with appropriate amounts of the test item (or reference material) being added.
- Pretreatment: none
- Initial biomass concentration: not reported
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 100 mg/L as CaCO3
- Test temperature:
- 21 °C
- pH:
- 6.9
- Nominal and measured concentrations:
- Nominal concentrations: 125, 250, 500, 1000 and 2000 mg test item/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: a) 500-mL conical flasks and b) 250-mL darkened glass Biological Oxygen Demand bottles
- Aeration: aerated with compressed air via narrow bore glass tubes at approximately 0.5 - 1.0 L per minute
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 3.9 suspended solids/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: laboratory tap water dechlorinated by passage through an activated carbon filter
- Total Hardness: 100 mg/L as CaCO3
OTHER TEST CONDITIONS
- Photoperiod: normal laboratory lighting
EFFECT PARAMETERS MEASURED: Oxygen consumption rates and percentage inhibition of respiration were measured after 30 minutes and 3 hours contact time
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 100 and 1000 mg test item/L in the first range-finding study and 1000 and 1800 mg test item/L in the second one.
- Results used to determine the conditions for the definitive study: significant inhibition of respiration was observed at 1000 and 1800 mg/L, but no significant effects on respiration was observed at 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 012.77 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 709 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 84 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: self-estimated using TRAP v1.30a
- Details on results:
- - The variation in respiration rates of the controls after 30 minutes contact time was ± 13 %.
- The variation in respiration rates of the controls after 3 hours contact time was ± 3 %.
- After 30 minutes contact time, no concentration tested resulted in 50 % inhibition, and therefore it was considered inappropriate to extrapolate the plot of inhibition against concentration to obtain an EC50 value after 30 minutes contact time. - Results with reference substance (positive control):
- After 30 minutes and 3 hours contact time, the EC50 values of 3,5-dichlorophenol were determined to be 14 mg/L and 9 mg/L, respectively.
- Reported statistics and error estimates:
- In order to calculate the inhibitory effect of the test item and the reference substance, the respiration rate was expressed as a percentage of the two control respiration rates. Percentage inhibition was plotted against concentration and the EC50 values were derived by inspection of the graph.
Any other information on results incl. tables
Table 1. Oxygen consumption rates and percentage inhibition values of tetraammineplatinum (2 +) hydrogencarbonate after 30 minutes contact time.
| Nominal concentration (mg/L) | O2 Consumption rates (mg O2/L/min) | % Inhibition | |
| Control | Replicate 1 | 0.40 | - |
| Replicate 2 | 0.31 | - | |
| Tetraammineplatinum (2 +) hydrogencarbonate | 125 | 0.38 | 7* |
| 250 | 0.35 | 1 | |
| 500 | 0.35 | 1 | |
| 1000 | 0.35 | 1 | |
| 2000 | 0.31 | 13 | |
| Reference substance (3,5-dichlorophenol) | 3.2 | 0.42 | 18* |
| 10 | 0.18 | 49 | |
| 32 | 0.09 | 75 |
* increase
Table 2. Oxygen consumption rates and percentage inhibition values of tetraammineplatinum (2 +) hydrogencarbonate after 3 hours contact time.
| Concentration (mg/L) | O2 Consumption rates (mg O2/L/min) | % Inhibition | |
| Control | Replicate 1 | 0.38 | - |
| Replicate 2 | 0.36 | - | |
| Tetraammine platinum (2 +) hydrogencarbonate | 125 | 0.36 | 3 |
| 250 | 0.31 | 16 | |
| 500 | 0.27 | 27 | |
| 1000 | 0.24 | 35 | |
| 2000 | 0.16 | 57 | |
| Reference substance (3,5-dichlorophenol) | 3.2 | 0.34 | 8 |
| 10 | 0.13 | 65 | |
| 32 | 0.06 | 84 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, the 3-hour EC50 for tetraammineplatinum (2+) hydrogencarbonate on the respiration of activated sewage sludge microorganisms was 1400 mg test item/L corresponding to 709 mg Pt/L. The 3h-EC10 value was self-estimated as 84 mg Pt/L (using TRAP v1.30a).
- Executive summary:
Tetrammineplatinum (2+) hydrogencarbonate was tested for toxicity towards activated sludge of a predominantly domestic sewage. The study is reliable without restrictions, being GLP-compliant and having followed the standard test guideline (OECD 209).
In order to investigate the influence of the test substance towards activated sewage sludge, the rate of respiration was determined after 30 minutes and 3 hours contact time. Two range-finding studies were initially performed at test concentrations of 100, 1000 mg/L and 1000, 1800 mg/L. Significant inhibition of respiration was observed at 1000 and 1800 mg/L, but no significant effects on respiration were found at 100 mg/L. Therefore, nominal test concentrations of 125, 250, 500, 1000 and 2000 mg test item/L were selected for the definitive study. The substance 3,5-dichlorophenol was used as positive control. Based on nominal concentrations, the 3-h EC50 for the test item was determined to be 1400 mg test item/L corresponding to 709 mg Pt/L. The 3h-EC10 value was self-estimated as 84 mg Pt/L (using TRAP v1.30a).
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