4-(tricyclo[5.2.1.02,6]dec-8-ylidene)butyraldehyde

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The study was conducted between 22 March 1991 and 19 April 1991.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP study conducted in compliance with OECD Guideline 301B (modified by used of sealed vessels for assessment of volatile substances) without any deviation.

Remarks:

All particular details relating to the study set-up and conduct are not fully presented in Final Report, but refer to internal Standard Operating Procedure documents proper to the testing laboratory.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

Modified by use of sealed vessels for assessment of volatile substances

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dupical

IUPAC Name:

Dupical

Constituent 2

Reference substance name:

R683

IUPAC Name:

R683

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Dupical (Q), code R683
- Substance type: colourless light floral mobile oily liquid
- Physical state: liquid
- Analytical purity: see confidential section
- Impurities (identity and concentrations): see confidential section
- Isomers composition: see confidential section
- Purity test date: see confidential section
- Lot/batch No.: see confidential section
- Expiration date of the lot/batch: see confidential section
- Storage condition of test material: room temperature in the dark

Specific details on test material used for the study:

Manufacturer's Code.: 631488
Sample Description: colourless light floral mobile oily liquid
Purity (non-GLP): 89.0%
2 Isomers: 43 & 46%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent from an unacclimatised activated sludge plant secondary effluent at Unilever Research Laboratory North, used at 10% by volume
- Storage conditions/Preparation of inoculum for exposure: Effluent was filtered through a Whatman filter paper to remove coarse particulate matter.
- Concentration of sludge: no data

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

10 mg/L

Based on:

other: nominal organic carbon

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: compliant with 1988 OECD Ring Test on the harmonisation of ready biodegradability tests with the following deviation: the ferric chloride stock solution contained 0.25g not 0.2g. The EDTA stock solution contained 0.4g as recommended in 1988 OECD Ring Test and not 0.5g as specified in draft SOP 15801. No effect on the results expected.
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 16-22°C on the period
- pH: no data
- pH adjusted: yes, 6.3
- Suspended solids concentration: no data
- Continuous darkness: not stated

TEST SYSTEM
- Number of culture flasks/concentration: 5, except 3 only for the inoculum control
- Method used to create aerobic conditions: stirring
- Method used to create anaerobic conditions: not applicable
- Measuring equipment: Ionics 555 Inorganic Carbon Analyser.
- Test performed in closed vessels due to significant volatility of test substance: yes
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: not applicable

SAMPLING
- Sampling frequency: on day numbers, 4, 6, 11, 14, 18, 21, 25 and 28
- Sampling method: Ionics 555 Inorganic Carbon Analyser.

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: no data

Results and discussion

Preliminary study:

No data

Test performance:

No data

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

5.8

Sampling time:

28 d

Details on results:

- % biodegradation on Day 28 = 5.8%, 95% confidence limit [3.3-8.4 %]

BOD5 / COD results

Results with reference substance:

no data

Any other information on results incl. tables

Table 1 Percentage biodegradation of test substance

Day No	% biodegradation(a)
	R683
4	6.1
6	-1.2
11	2.5
14	-0.1
18	-0.1
21	-2.8
25	2.9
28	5.8
95% confid. limit	3.3 - 8.4

(a)   Based on nominal carbon concentration assuming 100% purity of the named test substance.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Under the test conditions, the substance is considered as not readily biodegradable.

Executive summary:

The biodegradation of Dupical was studied using a sealed vessel that is a CO2 production test based on OECD guideline 301B and studies conducted by the OECD aimed at harmonizing the various tests of ready biodegradability. The test system is closed and is therefore suitable for the examination of volatile materials. Inoculum came from secondary effluent from an unacclimatised activated sludge plant at Unilever Research Laboratory North. Initial nominal concentration of the test substance was 10 mg/l organic carbon. Biodegradation of the test substance is recorded by the evolution of the CO2 during the study. Analysis of both the headspace gas and the liquid medium for CO2/DIC was performed.

Dupical undergoes 5.8% biodegradation after 28 days. Therefore, and in accordance with the OECD Guidelines principles, Dupical is considered as not readily biodegradable. Validity criteria are all met, the study is considered as acceptable for the endpoint.