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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

LC50 (96 h) = 3.70 mg/l.

Key value for chemical safety assessment

LC50 for freshwater fish:
3.7 mg/L

Additional information

No information was available on the substance itself, thus the assessment relied on a read across approach. Solvent Red 008 and Similar Substance 02 are chromium complexes with 2 ligands. In both cases, the 2 ligands have the same chemical structure, i.e. a phenyl ring connected via diazo bond to a phenyl pyrazole. These ligands show minor differences in the position of substituents, i.e. hydroxy and nitro groups, as well as in the presence of a sulfonate group in Similar Substance 02.

All information about the read across process is inserted in section 13.

In the key study, the acute lethal toxicity of Similar Substance 01 (purity > 80 %) to fish was investigated under semi-static exposure conditions over a period of 96 h at nominal concentrations of 0.939, 2.07, 4.55 and 10 mg/l with rainbow trout according to OECD guideline 203. There was evidence that the concentration of test substance was satisfactorily maintained throughout the test, thus effect levels were expressed using nominal test item concentrations.

All fish were dead at the nominal concentrations of 10 mg/l after 4 h of exposure; 5/7 fishes were dead at the nominal concentration of 4.55 mg/l after 96 h; no substance-related mortality was observed at the lower concetrations after 96 h of exposure. Toxic effects other than mortality in terms of reduced motility and dark pigmentation were observed starting from the concentration of 2.07 mg/l.

On these bases, median lethal concentration (LC50) at 96 h was estimated to be 3.70 mg/l.

In a previous study on rainbow trout exposed to Similar Substance 01 (purity ca. 80 %), concentrations of 2, 10 and 20 mg/l were tested. At 2 mg/l, no fishes died after 48 h, while all fishes were dead after 24 h at 10 and 20 mg/l. Therefore, LC50(48 h) was estimated to be 6.3 mg/l.

Overall, based on these findings,it was concluded that the substance interacts with aquatic environment and it is toxic for freshwater fish, showing LC50 values below 10 mg/l. Following a precautionary approach, the lowest LC50 value obtained in studies with Similar Substance 02, i.e. 3.70 mg/l, was taken into account in the decision on classification under CLP Regulation (EC 1272/2008).