3-methylbut-2-en-1-ol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

The study was conducted according to an internal BASF method. Test groups consisting of 5 animals/sex/group were treated with doses of an aqueous solution of the test substance applied intraperitoneally. After exposure, the animals were observed for a period of 14 d within which time signs of clinical toxicity as well as mortality were monitored and recorded. Animals found dead during the observation period were subjected to necropsy. At conclusion, the surviving animals were also sacrificed for the purpose of necropsy. The LD50 value was estimated on the basis of the observed mortalities.

GLP compliance:

no

Remarks:

GLP was not compulsory at the time the study was conducted.

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-2-en-1-ol

EC Number:

209-141-4

EC Name:

3-methylbut-2-en-1-ol

Cas Number:

556-82-1

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-2-en-1-ol

Details on test material:

- Name of test substance: 3-Methylbut-2-en-1-ol (Prenol)
- Analytical purity: 95 %

Test animals

Species:

mouse

Strain:

other: Kisslegg

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Average weight at study initiation: 28-38 g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: aqueous emulsion with tragacanth

Details on exposure:

The substance was applied  as intraperitoneal injections as aqueous emulsions in tragacanth at concentrations of 20, 8, 4 and 2 %. 

Doses:

172, 344, 430, 550, 688 and 1376 mg/kg bw.
The original values were reported as 0.2, 0.4, 0.5, 0.64, 0.8 and 1.6 ml/kg bw. The mg/kg bw doses were based on a density at 20 °C of 0.86 g/ml.

No. of animals per sex per dose:

5 animals per sex per dose.

Control animals:

no

Details on study design:

Animals were inspected for signs of pharmacologic or toxicologic effects during a 14 days post observation period.
Body weight was measured before dosing. 
At the end of the observation period survivors were sacrificed and necropsied as were animals that died.
The LD50 value was estimated (calculation method not mentioned in the raw  data).

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

413 mg/kg bw

Mortality:

Mortality:
- 1.6 ml/kg bw: 5males and 5 females within the first 24 hours
- 0.8 ml/kg bw: 0 males and 2 females after 24 h; 1 male and 3 females after 48 h; 5 males and 5 females after 7 days
- 0.64 ml/kg bw: 1 male and 0 females after 24 h; 3 males and 0 females after 48 h; 5 males and 3 females after 7 days; 5 males and 3 females after 14days
- 0.5 ml/kg bw: 4 males and 2 females after 7 days; 5 males and 2 females after 14 days
- 0.4 ml/kg bw: 0/5 (males), 0/5 (females) after 14 days
- 0.2 ml/kg bw: 0/5 (males), 0/5 (females) after 14 days

Clinical signs:

1.6 ml/kg bw:
Abdominal, lateral and dorsal position, dyspnoea, staggering

0.4 - 0.8 ml/kg bw:
Abdominal position, dyspnoea, staggering; after 7 days, animals were without clinical symptoms

Gross pathology:

Adhesions in the abdomen, other organs without abnormalities