Reaction products of sodium sulphide, sulphur, carbon disulphide and potassium hydroxide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation. Incubation carried out in sealed vessels due to volatility of CS2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

incubation in sealed vessels due to volatility of CS2

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

not available

Vehicle:

no

Details on test solutions:

not available

Test organisms (species):

Daphnia magna

Details on test organisms:

not available

Test type:

other: incubation in sealed vessels

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no data

Hardness:

225 mg CaCO3/L

Test temperature:

20 deg C

pH:

8.1

Dissolved oxygen:

Not reported

Salinity:

Not reported

Nominal and measured concentrations:

Not reported.

Details on test conditions:

Tests were carried out in sealed vessels to prevent evaporation of CS2. The complete composition of the test medium is given in Van Leeuwen and Maas, Environ, pollution A37: 105-115 (1985). Daphnids were fed with algal cells (Chlorella pyrenoidosa), 1E5 cells/mL.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.1 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: 95% C.I.: 1.9 - 2.2 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

55 mg/L

Nominal / measured:

estimated

Conc. based on:

other: EC# 944-003-3

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

See attached background material

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 for Daphnia magna for carbon disulphide (CS2) in a sealed system was 2.1 mg/L. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 55 mg/l.

Executive summary:

The static test in a sealed system was carried out to determine the toxicity of CS2 to Daphnia magna. The test fulfilled the requirements of OECD Guideline 202 but was not carried out in compliance with GLP. The 48-h EC50 for CS2 for Daphnia magna was 2.1 mg/L. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 55 mg/l.

Description of key information

The substance degrades rapidly at pH <11 to form highly volatile Carbon disulphide (CS2). In a static test with CS2 in a sealed system to determine the toxicity of CS2 to Daphnia magna, the 48-h EC50 for CS2 was 2.1 mg/l. The test fulfilled the requirements of OECD Guideline 202 but was not carried out in compliance with GLP. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 55 mg/l. The basis for this estimate is attached as background information in the supporting study (read-across to CS2).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

55 mg/L

Additional information

The substance degrades rapidly at pH <11 to form highly volatile Carbon disulphide (CS2). In a static test with CS2 in a sealed system to determine the toxicity of CS2 to Daphnia magna, the 48-h EC50 for CS2 was 2.1 mg/l. The test fulfilled the requirements of OECD Guideline 202 but was not carried out in compliance with GLP. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 55 mg/l. The basis for this estimate is attached as background information in the supporting study (read-across to CS2).