3-phenylpropionic acid

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: refer below principle

Principles of method if other than guideline:

The reduction of aromatic acid i.e 3-phenylpropionic acid by the hyperthermophilic organism Pyrococcus furiosus was investigated.

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC name: 3-phenylpropanoic acid
- Molecular formula: C9H10O2
- Molecular weight: 150.176 g/mol
- Smiles: c1(CCC(O)=O)ccccc1
- InChI: 1S/C9H10O2/c10-9(11)7-6-8-4-2-1-3-5-8/h1-5H,6-7H2,(H,10,11)
- Substance type: Organic
- Physical state: solid

Analytical monitoring:

yes

Details on sampling:

The substrate concentration was 1 mM before inoculation.

Vehicle:

not specified

Test organisms (species):

other: Pyrococcus furiosus

Details on inoculum:

P. furiosus (DSM 3638) was grown anaerobically at 90°C in a basic medium.Yeast extract, cysteine, vitamins, and trace elements were added a Potato starch (5 g/1) was used as a carbon and energy source. Cells were grown in 100 ml bottles containing 50 ml medium,which was flushed with N2/CO2 (80/20) before inoculation. During growth, the bottles were shaken continuously at 200 rev./min. The medium was inoculated with a 2% preculture.

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

25 h

Remarks on exposure duration:

experimental conditions occured for 18 - 25 h after

Hardness:

No data

Test temperature:

90 deg.C

pH:

6.8-7.0

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

1 mM which is equivalent to 150.1 mg/l

Details on test conditions:

The start of growth the pH was 6.8 - 7.0 and was not adjusted during the experiment.

Reference substance (positive control):

not specified

Key result

Duration:

25 h

Dose descriptor:

EC50

Effect conc.:

150.1 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: In batch conversions 3-phenylpropionic acid was reduced with yield 69%.

Yield of alcohols from aromatic acid i.e 3-phenylpropanoic acid reduced byP. furiosus in batch culture.

Substrate	Product	Yield (%)
3-Phenylpropionic acid	3-Phenyl-1-propanol	68.7 ±4.2

 

Validity criteria fulfilled:

not specified

Conclusions:

In batch conversions experiment the test chemical 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.

Executive summary:

The reduction of aromatic acid i.e 3-phenylpropionic acid by the hyperthermophilic organism Pyrococcus furiosus was investigated.

P. furiosus (DSM 3638) was grown anaerobically at 90°C in a basic medium.Yeast extract, cysteine, vitamins, and trace elements were added a Potato starch (5 g/1) was used as a carbon and energy source. Cells were grown in 100 ml bottles containing 50 ml medium,which was flushed with N2/CO2 (80/20) before inoculation. During growth, the bottles were shaken continuously at 200 rev./min. The medium was inoculated with a 2% preculture.

Analytical method used as HPLC on a Nucleosil 100 C18 5U(Alltech, Laarne, Belgium) column. A Waters 600 controller and pump and a Waters 996 photodiode array detector were used (all Millipore, Milford, USA).Methanol/1% acetic acid was used as eluent and the analysis was isocratic.

1mM substrate concentration was used in experiment which is equivalent to 150.1 mg/l.

In batch conversions 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.

Description of key information

In batch conversions experiment the test chemical 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

150.1 mg/L

Additional information

The reduction of aromatic acid i.e 3-phenylpropionic acid by the hyperthermophilic organism Pyrococcus furiosus was investigated.

P. furiosus (DSM 3638) was grown anaerobically at 90°C in a basic medium.Yeast extract, cysteine, vitamins, and trace elements were added a Potato starch (5 g/1) was used as a carbon and energy source. Cells were grown in 100 ml bottles containing 50 ml medium,which was flushed with N2/CO2 (80/20) before inoculation. During growth, the bottles were shaken continuously at 200 rev./min. The medium was inoculated with a 2% preculture.

Analytical method used as HPLC on a Nucleosil 100 C18 5U(Alltech, Laarne, Belgium) column. A Waters 600 controller and pump and a Waters 996 photodiode array detector were used (all Millipore, Milford, USA).Methanol/1% acetic acid was used as eluent and the analysis was isocratic.

1mM substrate concentration was used in experiment which is equivalent to 150.1 mg/l.

In batch conversions 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.