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Reaction mass of Tetrasodium 2-[{4-[{4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulfonatonaphthalen-1-yl}diazenyl]-7-sulfonatonaphthalen-1-yl}diazenyl]benzene-1,4-disulfonate and Tetrasodium 2-[{4-[{4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulfonatonaphthalen-1-yl}diazenyl]-6-sulfonatonaphthalen-1-yl}diazenyl]benzene-1,4-disulfonate
EC number: 916-837-8 | CAS number: -
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes
- Specific details on test material used for the study:
- None
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- The sample solutions were taken from the waterbath at various times and the pH of each solution recorded. The concentration of the sample solution was determined by high performance liquid chromatography (HPLC).
- Buffers:
- The test system consisted of sterile buffer solutions at pH’s 4, 7 and 9.
- Estimation method (if used):
- None
- Details on test conditions:
- Performance of the Test:
Preparation of the Test Solutions:
Sample solutions were prepared in stoppered glass flasks at a nominal concentration of 100 mg/L in the three buffer solutions. The test solutions were split into individual vessels for each data point. The solutions were shielded from light whilst maintained at the test temperature.
Preliminary Test / Tier:
Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5 °C for a period of 144 hours.
Tier:
Results from the Preliminary Test / Tier 1 showed it was necessary to undertake further testing at pH 4 with solutions being maintained at 60.0 ± 0.5 °C and 70.0 ± 0.5 °C, and extending the 50 °C test (Tier 1) to 744 hours.
Evaluation of Data:
The response factors of the standard peak areas (unit peak area per mg/L) were calculated using the following equation:
Equation 1
STDSTDCR = RF
where:
RF = response factor for the standard solution
RSTD = peak area for the standard solution
CSTD = concentration for the standard solution (mg/L)
Each sample solution concentration (g/L) was calculated using the following equation: - Duration:
- 144 h
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 100 mg/L
- Remarks:
- Tier - 1 - Preliminary test
- Duration:
- 744 h
- pH:
- 4
- Temp.:
- 70 °C
- Initial conc. measured:
- 100 mg/L
- Remarks:
- Tier 2
- Duration:
- 144 h
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 100 mg/L
- Remarks:
- Tier 1 - Preliminary test
- Duration:
- 144 h
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 100 mg/L
- Remarks:
- Tier 1 - Preliminary test
- Number of replicates:
- two
- Positive controls:
- no
- Negative controls:
- no
- Statistical methods:
- None
- Preliminary study:
- None
- Test performance:
- None
- Transformation products:
- not measured
- Details on hydrolysis and appearance of transformation product(s):
- None
- pH:
- 4
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0 h-1
- DT50:
- 198 d
- Remarks on result:
- other: Standard deviation not available
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- other: Standard deviation not available
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- other: Standard deviation not available
- Other kinetic parameters:
- None
- Details on results:
- None
- Results with reference substance:
- None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The estimated rate constant and half-lives at 25 °C of the test item are:
At pH 4 the estimated half-life at 25 °C was 198 days with estimated rate constant 0.000146
At pH 7 the estimated half-life at 25 °C was more than a year.
At pH 9 the estimated half-life at 25 °C was more than a year. - Executive summary:
The hydrolysis behaviour as a function of pH of FAT 40032/G has been evaluated according to OECD Guideline 111 and EU Method C.7.
The results are summarized below:
pH
Estimated rate constant (s-1) at 25 °C
Estimated half-life at 25 °C
4
1.46 x 10-6
198
7
-
> 1 year
9
-
> 1 year
Reference
Tier 2
The mean peak areas relating to the standard and sample solutions are shown in the following tables:
pH 7 - 40.0 ± 0.5 °C Tier 2 Testing
Solution |
Mean Peak Area |
Standard 102 mg/L |
8.6407 x 106 |
Standard 106 mg/L |
9.0462 x 106 |
Initial Sample A, pH 4 |
8.5429 x 106 |
Initial Sample B, pH 4 |
8.5465 x 106 |
| Standard 104 mg/L, pH 4 | 8.7344 X 106 |
| Standard 104 mg/L, pH 4 | 8.7569 x 106 |
| 71 hours Sample A, pH 4, 60 °C | 7.0521 x 106 |
| 71 hours Sample B, pH 4, 60 °C | 7.0509 x 106 |
| 71 hours Sample A, pH 4, 70 °C | 5.8135 x 106 |
| 71 hours Sample B, pH 4, 70 °C | 5.7857 x 106 |
| Standard 100 mg/L, pH 4 | 8.5041 x 106 |
Standard 102 mg/L, pH 4 |
8.5285 x 106 |
| 192 Hours Sample A, pH 4, 60 ºC | 5.8968 x 106 |
192 Hours Sample B, pH 4, 60 ºC |
5.8591 x 106 |
192 Hours Sample A, pH 4, 70 ºC |
4.0395 x 106 |
192 Hours Sample B, pH 4, 70 ºC |
3.9644 x 106 |
| Standard 99.8 mg/L, pH 4 | 8.2926 x 106 |
| Standard 103 mg/L, pH 4 | 8.6209 x 106 |
| 192 Hours Sample A, pH 4, 60 ºC | 5.2178 x 106 |
192 Hours Sample B, pH 4, 60 ºC |
5.2419 x 106 |
192 Hours Sample A, pH 4, 70 ºC |
3.2039 x 106 |
192 Hours Sample B, pH 4, 70 ºC |
3.1562 x 106 |
| Standard 102 mg/L, pH 4 | 8.9607 x 106 |
Standard 108 mg/L, pH 4 |
8.4690 x 106 |
240 Hours Sample A, pH 4, 60 ºC |
4.8335 x 106 |
240 Hours Sample B, pH 4, 60 ºC |
4.7509 x 106 |
240 Hours Sample A, pH 4, 70 ºC |
2.5438 x 106 |
240 Hours Sample B, pH 4, 70 ºC |
2.5137 x 106 |
| Standard 102 mg/L, pH 4 | 8.5285 x 106 |
Standard 101 mg/L, pH 4 |
8.4646 x 106 |
312 Hours Sample A, pH 4, 60 ºC |
4.0104 x 106 |
312 Hours Sample B, pH 4, 60 ºC |
4.0426 x 106 |
312 Hours Sample A, pH 4, 60 ºC |
1.7564 x 106 |
312 Hours Sample B, pH 4, 60 ºC |
1.8029 x 106 |
| Standard 101 mg/L, pH 4 | 8.4756 x 106 |
Standard 103 mg/L, pH 4 |
8.7408 x 106 |
360 Hours Sample A, pH 4, 60 ºC |
3.6122 x 106 |
360 Hours Sample B, pH 4, 60 ºC |
3.6103 x 106 |
360 Hours Sample A, pH 4, 60 ºC |
1.4099 x 106 |
360 Hours Sample B, pH 4, 60 ºC |
1.3736 x 106 |
Description of key information
The test substance FAT 40032/G has been determined to be hydrolytically stable, i.e., a half-life of greater than 1 year at 25 °C, at pH 7 and 9, whereas at pH 4 the estimated rate constant was 1.46 X 10 -6 with a half-life of 198 days.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 1 yr
- at the temperature of:
- 25 °C
Additional information
The hydrolysis behaviour as a function of pH of FAT 40032/G has been evaluated according to OECD Guideline 111 and EU Method C.7.
The results are summarized below:
pH | Estimated rate constant (s-1) at 25 °C | Estimated half-life at 25 °C |
4 | 1.46 x 10-6 | 198 |
7 | - | >1 year |
9 | - | >1 year |
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