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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
December 2016-March 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
impurity
Test material form:
liquid
Details on test material:
- Name of test material (as cited in the expert statement): Folenox
- Chemical name: 4,4,8,8-Tetramethyloctahydro-4a,7-methanonaphtho[1,8a-b]oxirene (IUPAC)
- Empirical formula: C15H24O
- Structural formula: See IUCLID section 1

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Sampling for analysis of test concentrations was done at 0h and 48h.

Test solutions

Vehicle:
no
Details on test solutions:
The study started with a combined limit/range-finding test. Twenty daphnids per concentration (four replicates, five daphnids per replicate) were exposed to a control and a WAF at 100 mg/L.
For the final test, test concentrations were WAFs at 0.10, 0.22, 0.46, 1.0, 2.2, 4.6 and 10 mg/L.
Samples taken from the WAFs at 1.0, 2.2, 4.6 and 10 mg/L were analyzed and showed measured initial concentrations of 0.68, 1.6, 3.1 and 7.3 mg/L, respectively. These concentrations remained stable during the test period and were 95-98% of initial at the end of the test. Given these results, the EC50 was calculated using the measured initial concentrations.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
180 mg/L expressed as CaCO3
Test temperature:
The temperature was continuously measured in a temperature control vessel. Temperature varied between 19 and 20°C during the test.
pH:
pH 7.7 +/- 0.3
Nominal and measured concentrations:
Nominal concentrations: WAFs at 0.10, 0.22, 0.46, 1.0, 2.2, 4.6 and 10 mg/L.
Measured initial concentrations: 0.68, 1.6, 3.1 and 7.3 mg/L (corresponding to WAFs at 1.0, 2.2, 4.6 and 10 mg/L, respectively).
Reference substance (positive control):
no
Remarks:
Negative control was used - test medium without test item or other additives. Reference test carried out 14-16 Nov 2016 to check sensitivity of test system used by the laboratory.

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.8 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The 48h-EC50was 1.8 mg/L based on measured initial concentrations (95% confidence interval between 1.6 and 2.7 mg/L).